Chato, a KRAB zinc-finger protein, regulates convergent extension in the mouse embryo.
Chato, a KRAB zinc-finger protein, regulates convergent extension in the mouse embryo.
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DOI：
10.1242/dev.022897
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Anderson KV
中科院分区：
文献类型：
--
作者：
García-García MJ;Shibata M;Anderson KV
在非洲爪哇和斑马鱼胚胎中，前后体轴的伸长依赖于会聚伸展，这一过程涉及极化的细胞运动，并受非规范的Wnt信号调节。控制小鼠胚胎轴伸长的机制还知之甚少。在这里，我们描述了ENU诱导的小鼠突变Chato，它导致妊娠中期停滞和收敛延伸突变体的缺陷，包括体轴缩短、体节内侧延伸和神经管开放。Chato突变破坏了Zfp568，Zfp568是一个Krüppel Associated Box(KRAB)结构域的锌指蛋白。形态计量学分析表明，小鼠野生型胚胎的最终内胚层在体节早期经历了导致会聚延伸的细胞重排，而在Chato胚胎中这些细胞重排失败。尽管非典范的Wnt信号对小鼠脊索和神经板的汇聚延伸很重要，但结果表明，Chato通过一个独立于非典范的Wnt信号的过程来调节所有胚胎组织中的体轴延长。
In Xenopus and zebrafish embryos, elongation of the anterior-posterior body axis depends on convergent extension, a process that involves polarized cell movements and is regulated by non-canonical Wnt signaling. The mechanisms that control axis elongation of the mouse embryo are much less well understood. Here, we characterize the ENU-induced mouse mutation chato, which causes arrest at midgestation and defects characteristic of convergent extension mutants, including a shortened body axis, mediolaterally extended somites and an open neural tube. The chato mutation disrupts Zfp568, a Krüppel Associated Box (KRAB) domain Zinc finger protein. Morphometric analysis reveals that the definitive endoderm of mouse wild-type embryos undergoes cell rearrangements that lead to convergent extension during early somite stages, and that those cell rearrangements fail in chato embryos. Although non-canonical Wnt signaling is important for convergent extension in the mouse notochord and neural plate, the results indicate that chato regulates body axis elongation in all embryonic tissues through a process that is independent of non-canonical Wnt signaling.
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影响因子：
5.3
作者：
Gebelein, B;Urrutia, R
通讯作者：
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影响因子：
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作者：
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作者：
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DOI：
10.1073/pnas.88.9.3608
发表时间：
1991-05-01
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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