Immunogenic Eimeria tenella glycosylphosphatidylinositol-anchored surface antigens (SAGs) induce inflammatory responses in avian macrophages.
Immunogenic Eimeria tenella glycosylphosphatidylinositol-anchored surface antigens (SAGs) induce inflammatory responses in avian macrophages.
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DOI：
10.1371/journal.pone.0025233
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Nathan S
中科院分区：
文献类型：
--
作者：
Chow YP;Wan KL;Blake DP;Tomley F;Nathan S
柔嫩艾美耳球虫第二代裂殖子至少表达19种糖基磷脂酰肌醇(GPI)锚定表面抗原(SAGs)，但这些蛋白在鸡体内刺激免疫反应的能力尚不清楚。10个SAG属于先前定义的两个多基因家族(A和B)，在大肠杆菌中以可溶性重组(R)融合蛋白的形式表达。用纯化的rSAGs处理鸡巨噬细胞，检测巨噬细胞亚硝酸盐生成、诱导型一氧化氮合酶(INOS)和一系列细胞因子的mRNA表达谱的变化。RSAG4、5和12可诱导巨噬细胞产生高水平的一氧化氮和IL-1β转录，这可能与柔嫩艾美耳球虫感染过程中观察到的炎症反应有关。同时，rSAG4、5和12处理抑制了IL-12和干扰素-γ的表达，而上调了IL-10的表达，提示在感染过程中，这些分子可能特异性地损害了细胞免疫的发展。综上所述，一些柔嫩艾美耳球虫SAG对鸡的天然免疫和体液免疫反应有不同的调节作用，而来自多基因家族A(尤其是rSAG 12)的SAG可能与柔嫩艾美耳球虫的致病作用更紧密地联系在一起，与内源性第二代阶段有关。
At least 19 glycosylphosphatidylinositol (GPI)-anchored surface antigens (SAGs) are expressed specifically by second-generation merozoites of Eimeria tenella, but the ability of these proteins to stimulate immune responses in the chicken is unknown. Ten SAGs, belonging to two previously defined multigene families (A and B), were expressed as soluble recombinant (r) fusion proteins in E. coli. Chicken macrophages were treated with purified rSAGs and changes in macrophage nitrite production, and in mRNA expression profiles of inducible nitric oxide synthase (iNOS) and of a panel of cytokines were measured. Treatment with rSAGs 4, 5, and 12 induced high levels of macrophage nitric oxide production and IL-1β mRNA transcription that may contribute to the inflammatory response observed during E. tenella infection. Concomitantly, treatment with rSAGs 4, 5 and 12 suppressed the expression of IL-12 and IFN-γ and elevated that of IL-10, suggesting that during infection these molecules may specifically impair the development of cellular mediated immunity. In summary, some E. tenella SAGs appear to differentially modulate chicken innate and humoral immune responses and those derived from multigene family A (especially rSAG 12) may be more strongly linked with E. tenella pathogenicity associated with the endogenous second generation stages.
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影响因子：
1.8
作者：
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