Sim1-expressing cells illuminate the origin and course of migration of the nucleus of the lateral olfactory tract in the mouse amygdala.
Sim1-expressing cells illuminate the origin and course of migration of the nucleus of the lateral olfactory tract in the mouse amygdala.
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Sim1表达细胞阐明了小鼠杏仁核外侧嗅束核的起源和迁移过程。
DOI：
10.1007/s00429-020-02197-1
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发表时间：
2021-03
影响因子：
3.1
通讯作者：
Puelles L
中科院分区：
文献类型：
--
作者：
Garcia-Calero E;López-González L;Martínez-de-la-Torre M;Fan CM;Puelles L
我们的报告集中在外侧嗅束核(NLOT)，这是一个接受嗅觉输入的杏仁浅核。与其表达Tbr1的第二层锥体细胞群(NLOT2)混合，存在表达Sim1的细胞，其胚胎起源和到达方式尚不清楚。我们用Sim1-ish和Sim1-tauLacZ小鼠品系研究了这个群体。翼状下丘脑起源于室旁区域，它早熟地表达SIM1。这一前体区在E10.5显示了表达Sim1的背侧延伸，穿过端脑柄，沿着末端沟，到达杏仁皮质的尾内侧端。我们认为这个表达Sim1的下丘脑-杏仁走廊(HYA)是下丘脑室旁区的一个外展部分，参与了表达Sim1的细胞的产生。从E13.5开始，表达SIM1的细胞通过HYA穿透杏仁后叶放射状单位，并在其中结合到表达Tbr1的早期迁移流，其内侧摆动穿过杏仁前基底外侧核(E15.5)，越过苍白质-苍白质下边界(E16.5)，并在E17.5形成杏仁前核内的NLOT2。我们的结论是，表达Tbr1的NLOT2细胞严格地出现在杏仁皮质后单位，涉及我们所讨论的各种必需的基因功能。我们的结果与Remedios等人关于NLOT2起源的实验数据是一致的。(NAT Neurosci 10：1141-1150,2007)，但我们不同意它们在背侧大脑皮层这个过程中的含义，观察到背侧大脑皮层远离杏仁核。
We focus this report on the nucleus of the lateral olfactory tract (NLOT), a superficial amygdalar nucleus receiving olfactory input. Mixed with its Tbr1-expressing layer 2 pyramidal cell population (NLOT2), there are Sim1-expressing cells whose embryonic origin and mode of arrival remain unclear. We examined this population with Sim1-ISH and a Sim1-tauLacZ mouse line. An alar hypothalamic origin is apparent at the paraventricular area, which expresses Sim1 precociously. This progenitor area shows at E10.5 a Sim1-expressing dorsal prolongation that crosses the telencephalic stalk and follows the terminal sulcus, reaching the caudomedial end of the pallial amygdala. We conceive this Sim1-expressing hypothalamo-amygdalar corridor (HyA) as an evaginated part of the hypothalamic paraventricular area, which participates in the production of Sim1-expressing cells. From E13.5 onwards, Sim1-expressing cells migrated via the HyA penetrate the posterior pallial amygdalar radial unit and associate therein to the incipient Tbr1-expressing migration stream which swings medially past the amygdalar anterior basolateral nucleus (E15.5), crosses the pallio-subpallial boundary (E16.5), and forms the NLOT2 within the anterior amygdala by E17.5. We conclude that the Tbr1-expressing NLOT2 cells arise strictly within the posterior pallial amygdalar unit, involving a variety of required gene functions we discuss. Our results are consistent with the experimental data on NLOT2 origin reported by Remedios et al. (Nat Neurosci 10:1141–1150, 2007), but we disagree on their implication in this process of the dorsal pallium, observed to be distant from the amygdala.
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影响因子：
3.8
作者：
Bardet, Sylvia M.;Martinez-de-la-Torre, Margaret;Puelles, Luis
通讯作者：
Puelles, Luis
影响因子：
3.1
作者：
Alonso, Antonia;Maria Trujillo, Carmen;Puelles, Luis
通讯作者：
Puelles, Luis
影响因子：
2.5
作者：
Bupesh, Munisamy;Abellan, Antonio;Medina, Loreta
通讯作者：
Medina, Loreta
影响因子：
2.5
作者：
Bishop, KM;Garel, S;O'Leary, DDM
通讯作者：
O'Leary, DDM
影响因子：
3.5
作者：
Fan, CM;Kuwana, E;TessierLavigne, M
通讯作者：
TessierLavigne, M