Fetomaternal Trafficking in the Mouse Increases as Delivery Approaches and Is Highest in the Maternal Lung1
Fetomaternal Trafficking in the Mouse Increases as Delivery Approaches and Is Highest in the Maternal Lung1
复制标题
随着分娩的临近，小鼠的胎儿母体贩运量增加，并且在母体肺中的贩运量最高1
作者：
Y. Fujiki;Kirby L. Johnson;H. Tighiouart;Inga Peter;D. Bianchi
摘要本研究的目的是更详细地了解健康妊娠小鼠胎母细胞运输的自然历史。我们的目标是确定怀孕期间最好的靶器官和天数，以便进一步从机制上研究胎儿细胞在母体疾病和伤害中的作用。C57BL/6J野生型处女(n=54)与同源增强型绿色荧光蛋白(EGFP)转基因雄性小鼠交配。孕期和产后处死雌性小鼠，用流式细胞仪和实时定量聚合酶链式反应分析8个器官和血液中是否存在胎儿GFP+细胞。母鼠的肺、肝和脾也用荧光立体显微镜进行了分析。胎儿GFP+细胞在胚胎第11天开始出现低频率，在胚胎第19天达到高峰，产后迅速下降。这些胎儿细胞动力学在所有被研究的小鼠中都具有显著的重复性。此外，胎儿细胞在母体器官中的分布是一致的，其中肺、肝、血和脾的浓度最高；这些在所有时间点都高度相关(P<；0.0001)。母肺中含有的胎儿细胞是其他任何器官的10到100倍，使用这三种技术，在这个器官中检测到的胎儿细胞数量是最一致和最具重复性的。体视显微镜显示，在肺内，胎儿细胞广泛且明显随机分布。使用小鼠模型，我们的数据表明，胎儿-母体细胞的运输是以可复制的模式发生的，是最大的近期分娩，并且对母体肺有偏爱。
Abstract The purpose of the study was to understand in more detail the natural history of fetomaternal cell trafficking in healthy pregnant mice. Our goal was to identify the best target organs and days during pregnancy for further mechanistic studies of the role of fetal cells in maternal disease and injury. C57BL/6J wild-type virgin females (n = 54) were mated with congenic enhanced green fluorescent protein (EGFP) transgenic males. During pregnancy and after delivery, female mice were euthanized, and eight organs and blood were analyzed for the presence of fetal GFP+ cells with flow cytometry and real-time quantitative PCR. Maternal lungs, liver, and spleen were also analyzed by fluorescent stereomicroscopy. Fetal GFP+ cells were first found at low frequency at Embryonic Day 11, increased to a maximum at Embryonic Day 19, and decreased rapidly postpartum. These fetal cell dynamics were significantly reproducible among all mice studied. In addition, there was a consistent distribution of fetal cells within maternal organs, with lung, liver, blood, and spleen having the greatest concentrations; these were highly correlated at all time points (P < 0.0001). Maternal lung contained 10- to 100-fold more fetal cells than any other organ, and using all three techniques, the number of fetal cells detected was the most consistent and reproducible in this organ. Stereomicroscopy showed that within the lung, fetal cells were widely and apparently randomly distributed. Using a murine model, our data demonstrate that fetomaternal cellular trafficking occurs in reproducible patterns, is maximal near term delivery, and has predilection for the maternal lung..
登录
查看更多内容
小鼠母体细胞微嵌合：使用实时 PCR 和体内成像进行分析。
影响因子：
3.6
作者：
Su,EricC;Johnson,KirbyL;Tighiouart,Hocine;Bianchi,DianaW
通讯作者：
Bianchi,DianaW
影响因子：
4.7
作者：
Chaudhuri, TR;Mountz, JM;Zinn, KR
通讯作者：
Zinn, KR
胎儿游离 DNA 在怀孕小鼠血浆中循环：与母胎贩运动物模型的相关性。
DOI：
10.1093/humrep/deh445
发表时间：
2004
期刊：
Human reproduction (Oxford, England)
影响因子：
--
作者：
Khosrotehrani,Kiarash;Wataganara,Tuangsit;Bianchi,DianaW;Johnson,KirbyL
通讯作者：
Johnson,KirbyL
影响因子：
20.3
作者：
Lambert, NC;Lo, YMD;Nelson, JL
通讯作者：
Nelson, JL
影响因子：
3.8
作者：
Lapaire, O.;Holzgreve, W.;Bianchi, D. W.
通讯作者：
Bianchi, D. W.