Functional analysis of the GRMZM2G174449 promoter to identify Rhizoctonia solani-inducible cis-elements in maize.
Functional analysis of the GRMZM2G174449 promoter to identify Rhizoctonia solani-inducible cis-elements in maize.
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GRMZM2G174449 启动子的功能分析，以鉴定玉米中立枯丝核菌诱导的顺式元件
DOI：
10.1186/s12870-017-1181-5
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发表时间：
2017-12-04
影响因子：
5.3
通讯作者：
Chu Z
中科院分区：
文献类型：
--
作者：
Yang F;Ding X;Chen J;Shen Y;Kong L;Li N;Chu Z
由水稻丝核菌引起的带状叶斑病是世界上大多数玉米和水稻种植区的一种严重的毁灭性病害。然而，感知病原体信号的分子机制知之甚少hosts.ResultsHere，我们确定了一个玉米立枯丝核菌诱导型启动子pGRMZM 2G 174449。缺失分析表明，-574到-455片段是pGRMZM 2G 174449应答R.生物信息学分析表明，该片段含有未知的病原菌诱导的顺式元件。此外，详细的定量分析表明，两个顺式元件，GCTGA在-567到-563区域和TATAT在-485到-481区域，是专门负责R。茄诱活性。一系列的点突变分析表明，这两个顺式元件分别具有NHWGN和DWYWT的保守基序。一种适合于BLSB抗性基因工程的茄属诱导型启动子。NHWGN和DWYWT是两个R。在pGRMZM 2G 174449应答R.索拉尼
BackgroundBanded leaf and sheath blight (BLSB), caused by the necrotrophic fungus Rhizoctonia solani, is a highly devastating disease in most maize and rice growing areas of the world. However, the molecular mechanisms of perceiving pathogen signals are poorly understood in hosts.ResultsHere, we identified a Rhizoctonia solani-inducible promoter pGRMZM2G174449 in maize. Deletion analysis showed that the −574 to −455 fragment was necessary for pGRMZM2G174449 in responding to R. solani and this fragment contained the unknown pathogen-inducible cis-elements according to the bioinformatics analysis. Furthermore, detailed quantitative assays showed that two cis-elements, GCTGA in the −567 to −563 region and TATAT in the −485 to −481 region, were specifically responsible for the R. solani-inducible activity. A series of point mutation analysis indicated that the two cis-elements have the conserved motifs of NHWGN and DWYWT, respectively.ConclusionOur results indicated that pGRMZM2G174449 is a good R. solani-inducible promoter suitable for genetic engineering of BLSB resistance. And NHWGN and DWYWT are two R. solani-inducible cis-elements that play important roles in pGRMZM2G174449 responding to R. solani.
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