CD40 signaling synergizes with TLR-2 in the BCR independent activation of resting B cells.
CD40 signaling synergizes with TLR-2 in the BCR independent activation of resting B cells.
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DOI：
10.1371/journal.pone.0020651
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Agrewala JN
中科院分区：
文献类型：
--
作者：
Jain S;Chodisetti SB;Agrewala JN
传统上，通过BCR发出的信号启动了激活和分化B细胞所需的一系列事件。我们报告了一种独立于BCR的刺激静息B(RB)细胞的替代方法，涉及TLR-2和CD40-对先天免疫和获得性免疫至关重要的分子。CD40激活TLR-2刺激的Rb细胞可显著增强其活化、增殖和分化。它还大大改善了钙离子流量、抗原摄取能力和B细胞激活T细胞的能力。Rb细胞存活率提高，表达活化诱导脱氨酶(AID)的细胞数量增加，表明类开关重组(CSR)。此外，我们还观察到RB细胞最佳刺激所需的激活率增加和阈值时间减少。这些结果与微阵列基因表达数据很好地印证了。这项研究为天然免疫分子和获得性免疫分子之间的协调提供了新的见解，以BCR非依赖的方式激活B细胞。这一策略可以被用来设计疫苗，以增强B细胞的激活和抗原呈递效率，导致更快和更好的免疫反应。
Conventionally, signaling through BCR initiates sequence of events necessary for activation and differentiation of B cells. We report an alternative approach, independent of BCR, for stimulating resting B (RB) cells, by involving TLR-2 and CD40 - molecules crucial for innate and adaptive immunity. CD40 triggering of TLR-2 stimulated RB cells significantly augments their activation, proliferation and differentiation. It also substantially ameliorates the calcium flux, antigen uptake capacity and ability of B cells to activate T cells. The survival of RB cells was improved and it increases the number of cells expressing activation induced deaminase (AID), signifying class switch recombination (CSR). Further, we also observed increased activation rate and decreased threshold period required for optimum stimulation of RB cells. These results corroborate well with microarray gene expression data. This study provides novel insights into coordination between the molecules of innate and adaptive immunity in activating B cells, in a BCR independent manner. This strategy can be exploited to design vaccines to bolster B cell activation and antigen presenting efficiency, leading to faster and better immune response.
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影响因子：
20.3
作者：
Lindsley, Robert Coleman;Thomas, Matthew;Allman, David
通讯作者：
Allman, David
影响因子：
32.4
作者：
Haas, KM;Hasegawa, M;Tedder, TF
通讯作者：
Tedder, TF
影响因子：
4.4
作者：
He, B;Qiao, XG;Cerutti, A
通讯作者：
Cerutti, A
影响因子：
4.4
作者：
Goetz, CA;Harmon, IR;Farrar, MA
通讯作者：
Farrar, MA
DOI：
10.1084/jem.159.3.881
发表时间：
1984-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ashwell JD;DeFranco AL;Paul WE;Schwartz RH
通讯作者：
Schwartz RH