Monoclonal antibodies specific for members of the genus Endotrypanum.

Monoclonal antibodies specific for members of the genus Endotrypanum.
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对内锥虫属成员具有特异性的单克隆抗体。

DOI:
10.1111/j.1550-7408.1989.tb05526.x
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发表时间:
1989
期刊:
The Journal of protozoology
影响因子:
--
通讯作者:
McMahon-Pratt,D
McMahon-Pratt,D
中科院分区:
--
文献类型:
--
作者:
Lopes,AH;McMahon-Pratt,D

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针对 Endotrypanum shaudinniorEndotrypanumsp 的膜富集制剂制备了 26 种单克隆抗体。前鞭毛体。其中 15 个单克隆抗体 (E1-E15) 仅与标准大肠杆菌菌株发生反应。肖丁尼,M6159。单克隆抗体 E16-E26 被认为是内锥虫特异性的;通过斑点印迹放射免疫测定,未检测到与锥虫科任何其他属(利什曼原虫、锥虫、细单胞菌、疱疹单胞菌)的交叉反应。通过间接免疫荧光测定,内锥虫特异性单克隆抗体识别的抗原似乎与寄生虫的表面相关。基于蛋白质印迹分析,通过对大肠杆菌菌株特异的单克隆抗体鉴定出分子量范围为24 kD至160 kD的4个抗原分子。肖丁尼,M6159。内锥菌属特异性单克隆抗体鉴定出分子量为 48 kD 的抗原以及表观分子量为 64-200 kD 的弥散迁移成分。
Twenty‐six monoclonal antibodies were produced against membrane‐enriched preparationsof Endotrypanum schaudinniorEndotrypanumsp. promastigotes. Fifteen of these monoclonal antibodies (E1‐E15) reacted only with the standard strain ofE. schaudinni, M6159. Monoclonal antibodies E16‐E26 were consideredEndotrypanumspecific; no cross reactivity was detected with any other genus of the family Trypanosomatidae(Leishmania, Trypanosoma, Leptomonas. HerpetomonasorCrithidia)by dot‐blot radioimmune assay. By indirect immunofluorescence assay, the antigens recognized byEndotrypanumspecific monoclonal antibodies appear to be associated with the surface of the parasite. Based on Western blot analysis, 4 antigenic molecules ranging in molecular weight from 24 kD to 160 kD were identified by monoclonal antibodies specific for the strain ofE. schaudinni, M6159. Monoclonal antibodies specific for the genusEndotrypanumidentified an antigen of molecular weight 48 kD as well as a diffuse component migrating with an apparent molecular weight of 64–200 kD.
区分巴西利什曼原虫亚种的单克隆抗体。
DOI: --
发表时间: 1982
影响因子: 4.4
作者:
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通过 125I 标记和免疫沉淀鉴定某些利什曼原虫种的交叉反应前鞭毛体细胞表面抗原
DOI: 10.1128/iai.43.2.637-643.1984
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白蛉(双翅目:Psychodidae)对二趾树懒(Edentata:Bradypodidae)的锥虫科的易感性。
DOI: --
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影响因子: 2.1
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Endotrypanum scaudinni(锥虫科)的研究贡献
DOI: --
发表时间: 1956
期刊:
影响因子: --
作者:
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利什曼原虫的两亲性硫酸化糖缀合物:用单克隆抗体进行表征。
DOI: 10.1002/j.1460-2075.1984.tb02130.x
发表时间: 1984
期刊: The EMBO journal
影响因子: --
作者:
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