The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns.
The SOD Gene Family in Tomato: Identification, Phylogenetic Relationships, and Expression Patterns.
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番茄中的 SOD 基因家族：鉴定、系统发育关系和表达模式
DOI：
10.3389/fpls.2016.01279
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发表时间：
2016
影响因子：
5.6
通讯作者：
Wan H
中科院分区：
文献类型：
--
作者：
Feng K;Yu J;Cheng Y;Ruan M;Wang R;Ye Q;Zhou G;Li Z;Yao Z;Yang Y;Zheng Q;Wan H
超氧化物歧化酶（sod）是一种重要的抗氧化酶，广泛存在于真核和原核细胞的细胞质、叶绿体和线粒体中，可以保护生物体免受不利条件引起的活性氧（ROS）的伤害。番茄（Solanum lycopersicum L.）是一种重要的经济作物，在世界各地都有种植。然而，非生物和生物胁迫严重阻碍了植物的生长发育，影响了作物的产量和质量。为了揭示SOD基因在不同胁迫下的潜在作用，我们对番茄SOD基因家族进行了系统分析，并在全基因组水平上分析了SlSOD基因在非生物胁迫下的表达模式。通过分析SlSOD基因家族的基因结构、保守基序、染色体分布、系统发育关系和表达模式来确定SlSOD基因家族的特征。结果表明，番茄中至少有9个SOD基因，包括4个Cu/ znsod、3个FeSODs和1个MnSOD，它们不均匀地分布在12条染色体上。对番茄和其他植物的SOD基因进行系统发育分析，结果表明，这些SOD基因在单子叶-双子叶分裂前就已经存在，具有较高的自举值。此外，在9个SlSOD基因的启动子中发现了许多响应不同胁迫的顺式元件。基于RNA-seq数据的基因表达分析显示，除SlSOD6和SlSOD8仅在幼果中表达外，大部分基因在所有被测组织中均有表达。微阵列数据分析显示，在盐胁迫和干旱胁迫条件下，SlSOD基因家族的大多数成员发生了改变。SlSOD基因的全基因组分析有助于阐明SlSOD基因在不同胁迫条件下的功能，并为进一步了解植物中SOD基因的进化关系提供信息。
Superoxide dismutases (SODs) are critical antioxidant enzymes that protect organisms from reactive oxygen species (ROS) caused by adverse conditions, and have been widely found in the cytoplasm, chloroplasts, and mitochondria of eukaryotic and prokaryotic cells. Tomato (Solanum lycopersicum L.) is an important economic crop and is cultivated worldwide. However, abiotic and biotic stresses severely hinder growth and development of the plant, which affects the production and quality of the crop. To reveal the potential roles of SOD genes under various stresses, we performed a systematic analysis of the tomato SOD gene family and analyzed the expression patterns of SlSOD genes in response to abiotic stresses at the whole-genome level. The characteristics of the SlSOD gene family were determined by analyzing gene structure, conserved motifs, chromosomal distribution, phylogenetic relationships, and expression patterns. We determined that there are at least nine SOD genes in tomato, including four Cu/ZnSODs, three FeSODs, and one MnSOD, and they are unevenly distributed on 12 chromosomes. Phylogenetic analyses of SOD genes from tomato and other plant species were separated into two groups with a high bootstrap value, indicating that these SOD genes were present before the monocot-dicot split. Additionally, many cis-elements that respond to different stresses were found in the promoters of nine SlSOD genes. Gene expression analysis based on RNA-seq data showed that most genes were expressed in all tested tissues, with the exception of SlSOD6 and SlSOD8, which were only expressed in young fruits. Microarray data analysis showed that most members of the SlSOD gene family were altered under salt- and drought-stress conditions. This genome-wide analysis of SlSOD genes helps to clarify the function of SlSOD genes under different stress conditions and provides information to aid in further understanding the evolutionary relationships of SOD genes in plants.
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影响因子：
4.3
作者：
Lee, Sang-Hoon;Ahsan, Nagib;Lee, Byung-Hyun
通讯作者：
Lee, Byung-Hyun
DOI：
10.1073/pnas.88.16.7381
发表时间：
1991-08-01
影响因子：
11.1
作者：
KELLER, GA;WARNER, TG;HALLEWELL, RA
通讯作者：
HALLEWELL, RA
影响因子：
1.8
作者：
Aydin, Semra;Buyuk, Ilker;Aras, Emine Sumer
通讯作者：
Aras, Emine Sumer
影响因子：
4.3
作者：
Asensio, Aaron C.;Gil-Monreal, Miriam;Fernando Moran, Jose
通讯作者：
Fernando Moran, Jose
影响因子：
3.1
作者：
Huang, Shengxiong;Gao, Yongfeng;Liu, Yongsheng
通讯作者：
Liu, Yongsheng