Cinnamomum cassia bark in two herbal formulas increases life span in Caenorhabditis elegans via insulin signaling and stress response pathways.
Cinnamomum cassia bark in two herbal formulas increases life span in Caenorhabditis elegans via insulin signaling and stress response pathways.
复制标题
DOI：
10.1371/journal.pone.0009339
复制
发表时间：
2010-02-22
期刊：
影响因子：
3.7
通讯作者：
Luo Y
中科院分区：
文献类型：
--
作者：
Yu YB;Dosanjh L;Lao L;Tan M;Shim BS;Luo Y
证明草药补充剂的功效和相应的作用模式是循证草药治疗的一个困难挑战。一个主要的障碍是草药制剂的复杂性，其中许多联合收割机组合多种草药，特别是当组合被认为对草药治疗的有效性至关重要时。这个问题可以通过使用现代方法和有效的动物模型来解决。在这项研究中，两个常用的传统草药配方，石泉大补汤（SQDB）和活络效灵丹（HLXL）进行了评估，使用生存试验和氧化应激生物标志物在一个良好的建立C。elegans衰老模型HLXL是一种十一草药配方，由畅销的传统草药配方改良而成，用于治疗关节炎关节疼痛。SQDB由十种草药组成，通常用于疲劳和能量，特别是在老年人中。我们在这里展示了SQDB显著地延长了C中的寿命。elegans衰老模型在所有试验的草药中，肉桂（Cinnamomum Cortex，CIN）和人参（Panax ginseng root，GS）两种草药显著延长了C.优雅的。SQDB和HLXL配方中的CIN通过调节多个长寿保证基因（包括参与胰岛素信号传导和应激反应途径的基因）来延长寿命。所有延长寿命的草药（SQDB，CIN和GS）也减弱了H2O2水平，并增强了小热休克蛋白的表达。此外，延长寿命的草药显着延迟人淀粉样蛋白β（Aβ）诱导的转基因C。表达人Aβ的线虫。这些结果验证了无脊椎动物模型的快速，系统的评价常用的中草药配方，并可能为设计未来的循证草药治疗（S）提供见解。
Proving the efficacy and corresponding mode of action of herbal supplements is a difficult challenge for evidence-based herbal therapy. A major hurdle is the complexity of herbal preparations, many of which combine multiple herbs, particularly when the combination is assumed to be vitally important to the effectiveness of the herbal therapy. This issue may be addressed through the use of contemporary methodology and validated animal models. In this study, two commonly used traditional herbal formulas, Shi Quan Da Bu Tang (SQDB) and Huo Luo Xiao Ling Dan (HLXL) were evaluated using a survival assay and oxidative stress biomarkers in a well-established C. elegans model of aging. HLXL is an eleven herb formula modified from a top-selling traditional herbal formula for the treatment of arthritic joint pain. SQDB consists of ten herbs often used for fatigue and energy, particularly in the aged. We demonstrate here that SQDB significantly extend life span in a C. elegans model of aging. Among all individual herbs tested, two herbs Cinnamomum cassia bark (Chinese pharmaceutical name: Cinnamomi Cortex, CIN) and Panax ginseng root (Chinese pharmaceutical name: Ginseng Radix, GS) significantly extended life span in C. elegans. CIN in both SQDB and HLXL formula extended life span via modulation of multiple longevity assurance genes, including genes involved in insulin signaling and stress response pathways. All the life-span-extending herbs (SQDB, CIN and GS) also attenuated levels of H2O2 and enhanced small heat shock protein expression. Furthermore, the life span-extending herbs significantly delayed human amyloid beta (Aβ)-induced toxicity in transgenic C. elegans expressing human Aβ. These results validate an invertebrate model for rapid, systematic evaluation of commonly used Chinese herbal formulations and may provide insight for designing future evidence-based herbal therapy(s).
登录
查看更多内容
影响因子：
4.7
作者：
Artal-Sanz, Marto;de Jong, Liesbeth;Tavernarakis, Nektarios
通讯作者：
Tavernarakis, Nektarios
影响因子：
64.8
作者：
Baur, Joseph A.;Pearson, Kevin J.;Sinclair, David A.
通讯作者：
Sinclair, David A.
DOI：
10.1093/gerona/62.12.1337
发表时间：
2007-12-01
影响因子：
5.1
作者：
Cao, Zhiming;Wu, YanJue;Luo, Yuan
通讯作者：
Luo, Yuan
影响因子：
5.8
作者：
Kirkham, S.;Akilen, R.;Tsiami, A.
通讯作者：
Tsiami, A.
影响因子：
56.9
作者：
Cohen, Ehud;Bieschke, Jan;Dillin, Andrew
通讯作者：
Dillin, Andrew