Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber-Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair.
Combining a Density Gradient of Biomacromolecular Nanoparticles with Biological Effectors in an Electrospun Fiber-Based Nerve Guidance Conduit to Promote Peripheral Nerve Repair.
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DOI：
10.1002/advs.202203296
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发表时间：
2023-02
期刊：
影响因子：
--
通讯作者：
Xue J
中科院分区：
文献类型：
--
作者：
Jin B;Yu Y;Lou C;Zhang X;Gong B;Chen J;Chen X;Zhou Z;Zhang L;Xiao J;Xue J
周围神经损伤是一个严重的医疗问题，手术和临床治疗选择有限。将多种引导信号整合在一个神经引导导管平台上，对促进轴突伸长和功能恢复具有重要意义。在这里，构建了一个多功能的NGC，通过结合有序的拓扑结构，生物大分子纳米颗粒的密度梯度和生物效应物的受控递送来促进神经再生，以分别提供拓扑，触针和生物线索。在排列的聚己内酯纳米纤维的表面上，沉积能够递送重组人酸性成纤维细胞生长因子的生物活性纳米颗粒的密度梯度。在梯度支架上，雪旺细胞的增殖得到促进，PC12细胞和背根神经节的神经突起的定向延伸在颗粒密度增加的方向上得到改善。在体内植入6周和12周以修复10 mm大鼠坐骨神经缺损后，NGC促进轴突伸长和髓鞘再生，不仅在解剖结构上而且在功能恢复上实现神经再生。总之，NGC为周围神经再生提供了有利的微环境，并有望实现接近自体移植物的神经修复效果。通过结合有序的拓扑结构、生物大分子纳米颗粒的密度梯度和生物效应物的受控递送来分别提供拓扑学、触觉和生物学线索，构建了多功能的基于静电纺丝纤维的NGC以促进神经再生。
Peripheral nerve injury is a serious medical problem with limited surgical and clinical treatment options. It is of great significance to integrate multiple guidance cues in one platform of nerve guidance conduits (NGCs) to promote axonal elongation and functional recovery. Here, a multi‐functional NGC is constructed to promote nerve regeneration by combining ordered topological structure, density gradient of biomacromolecular nanoparticles, and controlled delivery of biological effectors to provide the topographical, haptotactic, and biological cues, respectively. On the surface of aligned polycaprolactone nanofibers, a density gradient of bioactive nanoparticles capable of delivering recombinant human acidic fibroblast growth factor is deposited. On the graded scaffold, the proliferation of Schwann cells is promoted, and the directional extension of neurites from both PC12 cells and dorsal root ganglions is improved in the direction of increasing particle density. After being implanted in vivo for 6 and 12 weeks to repair a 10‐mm rat sciatic nerve defect, the NGC promotes axonal elongation and remyelination, achieving the regeneration of the nerve not only in anatomical structure but also in functional recovery. Taken together, the NGC provides a favorable microenvironment for peripheral nerve regeneration and holds great promise for realizing nerve repair with an efficacy close to autograft. A multi‐functional electrospun fiber‐based NGC is constructed to promote nerve regeneration by combining ordered topological structure, density gradient of biomacromolecular nanoparticles, and controlled delivery of biological effectors to provide the topographical, haptotactic, and biological cues, respectively.
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影响因子：
10
作者：
Lu Q;Zhang F;Cheng W;Gao X;Ding Z;Zhang X;Lu Q;Kaplan DL
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影响因子：
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