Neuronal regulation of astroglial morphology and proliferation in vitro.
Neuronal regulation of astroglial morphology and proliferation in vitro.
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DOI：
10.1083/jcb.100.2.384
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发表时间：
1985-02
期刊：
影响因子：
--
通讯作者：
Hatten ME
中科院分区：
文献类型：
--
作者：
Hatten ME
为了分析神经元和星形胶质细胞在中枢神经系统发育过程中的相互依赖性，已经开发了一种快速纯化出生后早期小脑神经元和星形胶质细胞并在体外重组它们的方法。神经元对星形胶质细胞形状和增殖的影响已经用先前用于描述小脑星形胶质细胞在神经元迁移和定位中的作用的体外模型系统进行了评价（哈滕，M. E、和R. K. H. Liem，1981，J. Cell Biol.，90：622-630;和哈滕，M. E、R. K. H. Liem，和C. A. Mason，1984，J. Cell Biol.，98：193-204。从出生后第3或4天的C57 B1/6 J小鼠小脑收获的小脑组织用胰蛋白酶解离成单细胞悬液，并用Percoll分级梯度分离富集的胶质和神经元组分。高度纯化的星形胶质细胞和神经元组分导致随后预种植的细胞上的聚赖氨酸包被的培养表面。在没有神经元的情况下，星形胶质细胞，通过对纯化的胶质纤维蛋白的抗血清染色鉴定，假设一个扁平的形状和迅速增殖。在没有星形胶质细胞，小脑神经元，通过抗血清对神经生长因子诱导的大的外部（NILE）糖蛋白和电子显微镜染色，形成细胞的再聚集体，有显着受损的轴突生长，并存活不佳。当纯化的神经元和分离的星形胶质细胞重组，星形胶质细胞的增殖显着放缓，扁平的形状表示在没有神经元转化成高度细长的配置文件，类似于胚胎形式的小脑星形胶质细胞。在神经元存在的较长时间（48-72小时）后，星形胶质细胞具有“Bergmann样”或“星形胶质细胞样”形状，并且神经元通常与它们相关。这些结果表明，神经元影响星形胶质细胞的分化。
To analyze the interdependence of neurons and astroglia during central nervous system development, a rapid method for purifying early postnatal cerebellar neurons and astroglia, and recombining them in vitro, has been developed. The influence of neurons on astroglial shape and proliferation has been evaluated with an in vitro model system previously used to describe the role of cerebellar astroglia in neuronal migration and positioning (Hatten, M. E., and R. K. H. Liem, 1981, J. Cell Biol., 90:622-630; and Hatten, M. E., R. K. H. Liem, and C. A. Mason, 1984, J. Cell Biol., 98:193-204. Cerebellar tissue harvested from C57Bl/6J mouse cerebellum on the third or fourth day postnatal was dissociated into a single cell suspension with trypsin, and enriched glial and neuronal fractions were separated with a step gradient of Percoll. Highly purified astroglial and neuronal fractions resulted from subsequently preplanting the cells on a polylysine-coated culture surface. In the absence of neurons, astroglia, identified by staining with antisera raised against purified glial filament protein, assumed a flattened shape and proliferated rapidly. In the absence of astroglia, cerebellar neurons, identified by staining with antisera raised against the nerve growth factor-inducible large external (NILE) glycoprotein and by electron microscopy, formed cellular reaggregates, had markedly impaired neurite outgrowth, and survived poorly. When purified neurons and isolated astroglia were recombined, astroglial proliferation slowed markedly and the flattened shape expressed in the absence of neurons transformed into highly elongated profiles that resembled embryonic forms of cerebellar astroglia. After longer periods (48-72 h) in the presence of neurons, astroglia had "Bergmann-like" or "astrocyte-like" shapes and neurons commonly associated with them. These results suggest that neurons influence the differentiation of astroglia.
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DOI：
10.1083/jcb.85.3.890
发表时间：
1980-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
McCarthy KD;de Vellis J
通讯作者：
de Vellis J
DOI：
10.1083/jcb.98.1.193
发表时间：
1984-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hatten ME;Liem RK;Mason CA
通讯作者：
Mason CA
影响因子：
2.5
作者：
ALTMAN, J;BAYER, SA
通讯作者：
BAYER, SA
影响因子：
64.8
作者：
MUDGE, AW
通讯作者：
MUDGE, AW
DOI：
10.1007/bf01181580
发表时间：
1976-01-01
期刊：
JOURNAL OF NEUROCYTOLOGY
影响因子：
--
作者：
DELCERRO, M;SWARZ, JR
通讯作者：
SWARZ, JR