Phage nanofibers induce vascularized osteogenesis in 3D printed bone scaffolds.
Phage nanofibers induce vascularized osteogenesis in 3D printed bone scaffolds.
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DOI：
10.1002/adma.201400154
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发表时间：
2014-08-06
影响因子：
29.4
通讯作者：
Mao, Chuanbin
中科院分区：
文献类型：
--
作者：
Wang, Jianglin;Yang, Mingying;Zhu, Ye;Wang, Lin;Tomsia, Antoni P.;Mao, Chuanbin
自然骨是一种高度血管化的组织，依靠血管及时供应血液和营养来维持骨骼的完整性。[1，2]因此，在骨再生中，新形成的骨组织应该被血管化，以修复骨缺损。[1，3]已经证实，血管生成(血管形成)可以促进成骨(新骨形成)。[1，4]然而，目前的骨组织工程策略往往无法产生具有高密度血管的新骨。[5]已经提出了几种增强血管形成的策略，包括设计一种3D仿生支架；[6]输送血管生成生长因子如血管内皮生长因子[7]或使用高效细胞来源如干细胞或成熟血管细胞。[8]然而，这些方法在增加新骨中的血管形成方面仅取得了有限的成功。为了设计一种能够支持细胞生长和诱导血管生成的材料，应该考虑血管生成的生物学机制。众所周知，血管生成依赖于细胞-细胞和细胞-基质的黏附相互作用。[9，10]整合素是细胞黏附分子。自然界已经发现了20多种整合素，其中至少有6种包括αvβ3、αvβ5、α5β1、αvβ1、α2β1和α1β1在血管再生中起关键作用。[11]其中，含有αv的整合素在创伤愈合过程中高表达于血管内皮细胞(EC)，而不是在失活的EC中，因此对血管再生至关重要。
Natural bone is a highly vascularized tissue that relies on blood vessels for the timely supply of blood and nutrition to maintain skeletal integrity.[1, 2] Thus in bone regeneration the newly formed bone tissue to heal bone defect should be vascularized.[1, 3] It has been established that angiogenesis (blood vessel formation) can promote osteogenesis (new bone formation).[1, 4] However, current bone tissue engineering strategies often fail to produce new bones with high densities of blood vessels.[5] Several strategies have been proposed to enhance vascularization, including the design of a 3D biomimetic scaffold;[6] delivery of angiogenic growth factors such as vascular endothelial growth factor (VEGF);[7] or the use of highly potent cell sources such as stem cells or mature vascular cells.[8] Nonetheless, these approaches have achieved only limited success in increasing vascularization in new bone.To design a material that can support cell growth and induce angiogenesis, biological mechanisms of angiogenesis should be considered. It is known that angiogenesis depends on the adhesive cell-cell and cell-matrix interactions.[9, 10] Integrins are cell adhesion molecules. More than 20 integrins have been discovered in nature, out of which at least six including αvβ3, αvβ5, α5β1, αvβ1, α2β1 and α1β1 play a crucial role in blood vessel regeneration.[11] Among them, the integrins containing αv are highly expressed in the activated endothelial cells (EC)(not in inactivated EC) in blood vessels during wound healing and thus are vital for blood vessel regeneration.[12] Because such integrins
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影响因子：
64.8
作者：
Pasqualini, R;Ruoslahti, E
通讯作者：
Ruoslahti, E
影响因子：
16.6
作者：
Mao, Chuanbin;Wang, Fuke;Cao, Binrui
通讯作者：
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影响因子：
56.9
作者：
BROOKS, PC;CLARK, RAF;CHERESH, DA
通讯作者：
CHERESH, DA
影响因子：
11.2
作者：
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通讯作者：
Thomas, Christian
影响因子：
16.6
作者：
Mao, Chuanbin;Liu, Aihua;Cao, Binrui
通讯作者：
Cao, Binrui