3D-printed bi-layered polymer/hydrogel construct for interfacial tissue regeneration in a canine model.
3D-printed bi-layered polymer/hydrogel construct for interfacial tissue regeneration in a canine model.
复制标题
DOI：
10.1016/j.dental.2022.06.020
复制
发表时间：
2022-08
期刊：
影响因子：
5
通讯作者：
Tayebi, Lobat
中科院分区：
文献类型：
--
作者：
Jamalpour, Mohammad Reza;Yadegari, Amir;Vahdatinia, Farshid;Amirabad, Leila Mohammadi;Jamshidi, Shokoofeh;Shojaei, Setareh;Shokri, Abbas;Moeinifard, Erfan;Omidi, Meisam;Tayebi, Lobat
关键词：
在硬组织和软组织的界面处应用再生策略存在并发症，这是由于可以在不同部位容纳不同细胞类型的结构的有限设计。该问题源于不同材料（例如聚合物和水凝胶）的粘附方面的挑战，这些材料可以适用于再生不同的组织，例如骨和软组织。本文提出了一种新的混合结构的设计，其中聚合物（聚己内酯（PCL））膜牢固地粘附到氢（明胶）层。使用3D打印制造具有限定尺寸和孔隙率的PCL膜。使用氨解程序将明胶层附着到PCL膜。我们已经研究了这种结构的应用引导骨再生（GBR）作为一个典型的外科再生程序的口腔在骨和软组织的界面。对犬胫骨骨缺损进行了完整的体外和体内研究。进行纤维化形态测量和骨形态测量评价的组织学分析，以及骨纤维化组织学分级和CBCT成像。膜的化学和形态学研究证明，明胶均匀地附着到胺解的PCL膜。体外和体内研究表明，膜的生物相容性，机械稳定性，和屏障功能的GBR应用。体外实验表明，该膜具有促进成骨和骨缺损再生的作用。结果表明，膜组的平均骨密度约为对照组的3倍。所制备的3D打印混合明胶/PCL双层膜可以是界面组织工程的良好候选物，并且是用于GBR手术的有前途的膜。
There are complications in applying regenerative strategies at the interface of hard and soft tissues due to the limited designs of constructs that can accommodate different cell types in different sites. The problem originates from the challenges in the adhesion of dissimilar materials, such as polymers and hydrogels, that can be suitable for regenerating different tissues such as bone and soft tissues. This paper presents a design of a new hybrid construct in which a polymer (polycaprolactone (PCL)) membrane firmly adheres to a layer of hydrogen (gelatin). PCL membranes with defined size and porosity were fabricated using 3D printing. The gelatin layer was attached to the PCL membranes using the aminolysis procedure. We have examined this construct for the application of Guided Bone Regeneration (GBR) as a typical surgical regenerative procedure of the oral cavity at the interface of bone and soft tissue. Complete in vitro and in vivo investigations on canine tibia bone defects have been performed. Histological analyses for fibrosis morphometric and bone morphometric evaluation, as well as bone-fibrosis histological grading and CBCT imaging, were conducted. Chemical and morphological studies of the membrane proved that gelatin was uniformly attached to the aminolyzed PCL membranes. The in vitro and in vivo studies indicated the membrane’s biocompatibility, mechanical stability, and barrier function for the GBR application. Furthermore, in vitro study showed that the membranes could improve osteogenesis and the regeneration of bone defects. The results illustrated that the mean bone density in the membrane groups was about three times more than that of the control group. The fabricated 3D-printed hybrid Gelatin/PCL bi-layered membrane can be a good candidate for interfacial tissue engineering and a promising membrane for GBR procedure.
登录
查看更多内容
影响因子：
9.7
作者：
Elgali, Ibrahim;Turri, Alberto;Omar, Omar
通讯作者：
Omar, Omar
影响因子：
4.3
作者：
Gielkens, Pepijn F. M.;Schortinghuis, Jurjen;Bos, Ruud R. M.
通讯作者：
Bos, Ruud R. M.
影响因子：
8.3
作者：
Dang, Hoang Phuc;Vaquette, Cedryck;Tran, Phong A.
通讯作者：
Tran, Phong A.
影响因子：
4.3
作者：
Kohal, RJ;Trejo, PM;Caffesse, RG
通讯作者：
Caffesse, RG
影响因子：
14
作者：
Ji, Wei;Yang, Fang;Jansen, John A.
通讯作者：
Jansen, John A.