Spatial distribution and characterization of non-apical progenitors in the zebrafish embryo central nervous system
Spatial distribution and characterization of non-apical progenitors in the zebrafish embryo central nervous system
复制标题
斑马鱼胚胎中枢神经系统非顶端祖细胞的空间分布和特征
作者：
Rebecca McIntosh;Joseph Norris;J. Clarke;Paula Alexandre
对非顶端祖细胞（NAPs）的研究主要限于发育中的哺乳动物皮层。它们被假定为产生神经元数量的增加，而神经元数量的增加是哺乳动物大脑扩张的基础。最近，也有报道称，非哺乳动物物种的视网膜和中枢神经系统中存在NAP;然而，在后者中，NAP的特征仍然很差。在这里，我们表征NAP的位置沿着斑马鱼胚胎发育的中枢神经系统，并确定其细胞和分子特征和更新能力。我们在斑马鱼胚胎的脊髓、后脑和端脑中发现了一小部分NAP。实时成像分析显示端脑中至少有两种类型的有丝分裂行为：一种NAP亚型在分裂过程中保留顶端附着，而另一种则在与顶端表面断开的亚顶端位置分裂。在脊髓中观察到的所有NAP在有丝分裂前失去了顶端接触。这些NAP表达HuC并从单个分裂中产生两个神经元。Notch活性的操纵揭示了脊髓中的神经元和NAP使用类似的调节机制。这项工作表明，斑马鱼中的大多数脊髓NAP与哺乳动物大脑中的基底祖细胞具有共同的特征。
Studies of non-apical progenitors (NAPs) have been largely limited to the developing mammalian cortex. They are postulated to generate the increase in neuron numbers that underlie mammalian brain expansion. Recently, NAPs have also been reported in the retina and central nervous system of non-mammalian species; in the latter, however, they remain poorly characterized. Here, we characterize NAP location along the zebrafish central nervous system during embryonic development, and determine their cellular and molecular characteristics and renewal capacity. We identified a small population of NAPs in the spinal cord, hindbrain and telencephalon of zebrafish embryos. Live-imaging analysis revealed at least two types of mitotic behaviour in the telencephalon: one NAP subtype retains the apical attachment during division, while another divides in a subapical position disconnected from the apical surface. All NAPs observed in spinal cord lost apical contact prior to mitoses. These NAPs express HuC and produce two neurons from a single division. Manipulation of Notch activity reveals that neurons and NAPs in the spinal cord use similar regulatory mechanisms. This work suggests that the majority of spinal NAPs in zebrafish share characteristics with basal progenitors in mammalian brains.
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影响因子：
11.4
作者：
Nonaka-Kinoshita, Miki;Reillo, Isabel;Artegiani, Benedetta;Angeles Martinez-Martinez, Maria;Nelson, Mark;Borrell, Victor;Calegari, Federico
通讯作者：
Calegari, Federico
影响因子：
16.6
作者：
Pilz, Gregor-Alexander;Shitamukai, Atsunori;Reillo, Isabel;Pacary, Emilie;Schwausch, Julia;Stahl, Ronny;Ninkovic, Jovica;Snippert, Hugo J.;Clevers, Hans;Godinho, Leanne;Guillemot, Francois;Borrell, Victor;Matsuzaki, Fumio;Goetz, Magdalena
通讯作者：
Goetz, Magdalena
影响因子：
56.9
作者：
Florio, Marta;Albert, Mareike;Huttner, Wieland B.
通讯作者：
Huttner, Wieland B.
Trnp1 通过控制径向胶质细胞命运来调节哺乳动物大脑皮层的扩张和折叠
影响因子：
64.5
作者：
Stahl R;Walcher T;De Juan Romero C;Pilz GA;Cappello S;Irmler M;Sanz-Aquela JM;Beckers J;Blum R;Borrell V;Götz M
通讯作者：
Götz M