Expression of Pax-3 is initiated in the early neural plate by posteriorizing signals produced by the organizer and by posterior non-axial mesoderm.
Expression of Pax-3 is initiated in the early neural plate by posteriorizing signals produced by the organizer and by posterior non-axial mesoderm.
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Pax-3 的表达是通过组织器和后非轴中胚层产生的后置信号在早期神经板中启动的。
作者：
A. Bang;N. Papalopulu;C. Kintner;M. Goulding
Pax-3是一个在背侧和后神经管中特异表达的配对型同源异型盒基因。我们已经调查了诱导的相互作用，启动Pax-3转录表达的早期神经板。我们提出了几条线的证据，支持一个模型，其中Pax-3的表达是由信号，posteriorize的神经轴，然后二次限制背侧响应背腹图案信号。首先，在鸡和非洲爪蟾原肠胚的Pax-3表达的发病发生在注定成为后中枢神经系统的地区。第二，亨森结和神经板下的后非轴向中胚层与假定的前神经板外植体结合时诱导Pax-3表达。与此相反，假定前神经板外植体不能表达Pax-3的背侧信号从表皮外胚层。第三，在异种外植体重组测定与非洲爪蟾动物帽（外胚层）作为响应组织，晚期，但不是早期，亨森氏结诱导Pax-3的表达。鸡后非轴向中胚层也诱导Pax-3，只要动物帽通过用noggin处理而神经化。最后，我们表明，假定的posteriorizing因素，视黄酸和bFGF，诱导Pax-3神经化的动物帽。然而，阻断实验与显性抑制性FGF受体和显性抑制性视黄酸受体表明，Pax-3诱导活动所产生的亨森氏结和后部非轴向中胚层不严格依赖于FGF或视黄酸。
Pax-3 is a paired-type homeobox gene that is specifically expressed in the dorsal and posterior neural tube. We have investigated inductive interactions that initiate Pax-3 transcript expression in the early neural plate. We present several lines of evidence that support a model where Pax-3 expression is initiated by signals that posteriorize the neuraxis, and then secondarily restricted dorsally in response to dorsal-ventral patterning signals. First, in chick and Xenopus gastrulae the onset of Pax-3 expression occurs in regions fated to become posterior CNS. Second, Hensen's node and posterior non-axial mesoderm which underlies the neural plate induce Pax-3 expression when combined with presumptive anterior neural plate explants. In contrast, presumptive anterior neural plate explants are not competent to express Pax-3 in response to dorsalizing signals from epidermal-ectoderm. Third, in a heterospecies explant recombinant assay with Xenopus animal caps (ectoderm) as a responding tissue, late, but not early, Hensen's node induces Pax-3 expression. Chick posterior non-axial mesoderm also induces Pax-3, provided that the animal caps are neuralized by treatment with noggin. Finally we show that the putative posteriorizing factors, retinoic acid and bFGF, induce Pax-3 in neuralized animal caps. However, blocking experiments with a dominant-inhibitory FGF receptor and a dominant-inhibitory retinoic acid receptor suggest that Pax-3 inductive activities arising from Hensen's node and posterior non-axial mesoderm do not strictly depend on FGF or retinoic acid.
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两栖动物中枢神经系统发育过程中前后模式的平面和垂直感应。
DOI：
10.1002/neu.480241003
发表时间：
1993
期刊：
Journal of neurobiology
影响因子：
--
作者：
Doniach,T
通讯作者：
Doniach,T
Pax3 对底板分化的抑制：来自转基因小鼠异位表达的证据。
DOI：
10.1242/dev.122.8.2555
发表时间：
1996
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Tremblay,P;Pituello,F;Gruss,P
通讯作者：
Gruss,P
影响因子：
4.6
作者：
Mary E. Dickinson;M. Selleck;Andrew P. McMahon;M. Bronner‐Fraser
通讯作者：
Mary E. Dickinson;M. Selleck;Andrew P. McMahon;M. Bronner‐Fraser
通过局部注射荧光组织化学标记物（外胚层衍生物）来绘制鸟类外胚层的命运图。
DOI：
10.1002/jez.1402550309
发表时间：
1990
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
Schoenwolf,GC;Sheard,P
通讯作者：
Sheard,P
影响因子：
4.6
作者：
M. Selleck;M. Bronner‐Fraser
通讯作者：
M. Selleck;M. Bronner‐Fraser