Cytogenetic identification and molecular marker development for the novel stripe rust-resistant wheat-Thinopyrum intermedium translocation line WTT11.
Cytogenetic identification and molecular marker development for the novel stripe rust-resistant wheat-Thinopyrum intermedium translocation line WTT11.
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DOI：
10.1007/s42994-021-00060-3
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发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Li, Zhensheng
中科院分区：
文献类型：
--
作者：
Yang, Guotang;Zheng, Qi;Hu, Pan;Li, Hongwei;Luo, Qiaoling;Li, Bin;Li, Zhensheng
关键词：
条锈病是由小麦条锈菌（Puccinia striiformis f.）小麦黑胫病（Pst）是小麦的主要病害之一。国际吧小偃78829是由普通小麦与中间偃麦草杂交选育而成的部分双二倍体，对小麦条锈病免疫。为了将这一优良种质资源的抗病基因转移到小麦上，利用花粉辐射法获得了小麦易位系WTT 11，并对其进行了抗条锈性鉴定。一个新的抗条锈病基因座来源于Th.通过连锁和诊断标记分析证实了中间型。分子细胞遗传学分析表明WTT 11携带TTh·2DL易位。1B的断裂点位于95.5MB处，根据小麦660 K单核苷酸多态性（SNP）阵列分析，发现外源片段与小麦组第6和第7染色体同源。10个先前开发的基于PCR的标记被证实可以快速追踪WTT 11的外来片段，并且开发了20个竞争等位基因特异性PCR（KASP）标记以使Th的基因分型成为可能。中间小麦和普通小麦。通过连续两季的农艺性状评价，发现WTT 11具有株高低、主穗长等优良农艺性状，可用于小麦品种的改良。作为一种新的抗病基因资源，该基因座可能在小麦抗病育种中发挥重要作用。在线版本包含补充材料，可通过10.1007/s42994-021-00060-3获得。
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Xiaoyan 78829, a partial amphidiploid developed by crossing common wheat with Thinopyrum intermedium, is immune to wheat stripe rust. To transfer the resistance gene of this excellent germplasm resource to wheat, the translocation line WTT11 was produced by pollen irradiation and assessed for immunity to stripe rust races CYR32, CYR33 and CYR34. A novel stripe rust-resistance locus derived from Th. intermedium was confirmed by linkage and diagnostic marker analyses. Molecular cytogenetic analyses revealed that WTT11 carries a TTh·2DL translocation. The breakpoint of 1B was located at 95.5 MB, and the alien segments were found to be homoeologous to wheat-group chromosomes 6 and 7 according to a wheat660K single-nucleotide polymorphism (SNP) array analysis. Ten previously developed PCR-based markers were confirmed to rapidly trace the alien segments of WTT11, and 20 kompetitive allele-specific PCR (KASP) markers were developed to enable genotyping of Th. intermedium and common wheat. Evaluation of agronomic traits in two consecutive crop seasons uncovered some favorable agronomic traits in WTT11, such as lower plant height and longer main panicles, that may be applicable to wheat improvement. As a novel genetic resource, the new resistance locus may be useful for wheat disease-resistance breeding. The online version contains supplementary material available at 10.1007/s42994-021-00060-3.
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影响因子：
3.1
作者：
Fedak, G;Chen, Q;Armstrong, KW
通讯作者：
Armstrong, KW
影响因子：
3.2
作者：
CHEN, XM;JONES, SS;LINE, RF
通讯作者：
LINE, RF
影响因子：
2.3
作者：
Chicaiza, O;Khan, IA;Dubcovsky, J
通讯作者：
Dubcovsky, J
通过全基因组渗入小麦的脑膜膜染色体表征。
DOI：
10.1007/s00122-017-3009-y
发表时间：
2018-03
期刊：
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
影响因子：
--
作者：
Grewal S;Yang C;Edwards SH;Scholefield D;Ashling S;Burridge AJ;King IP;King J
通讯作者：
King J
影响因子：
2
作者：
Ardalani, Somayeh;Mirzaghaderi, Ghader;Badakhshan, Hedyeh
通讯作者：
Badakhshan, Hedyeh