Wuji Wan Formula Ameliorates Diarrhea and Disordered Colonic Motility in Post-inflammation Irritable Bowel Syndrome Rats by Modulating the Gut Microbiota.
Wuji Wan Formula Ameliorates Diarrhea and Disordered Colonic Motility in Post-inflammation Irritable Bowel Syndrome Rats by Modulating the Gut Microbiota.
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乌鸡丸方通过调节肠道菌群改善炎症后肠易激综合征大鼠的腹泻和结肠蠕动紊乱
DOI：
10.3389/fmicb.2017.02307
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发表时间：
2017
影响因子：
5.2
通讯作者：
Qiang W
中科院分区：
文献类型：
--
作者：
Chen Y;Xiao S;Gong Z;Zhu X;Yang Q;Li Y;Gao S;Dong Y;Shi Z;Wang Y;Weng X;Li Q;Cai W;Qiang W
新的证据表明，肠道微生物区系有助于炎症性肠易激综合征(PI-IBS)的治疗。我们之前的研究已经证明，中国方剂无极丸具有缓解PI-IBS大鼠腹痛和腹泻的能力。然而，其潜在的机制以及肠道微生物区系是否介导了无极丸对PI-IBS的影响还知之甚少。因此，本研究的目的是确定无极丸是否通过改变肠道微生物区系来减轻PI-IBS。用4%醋酸灌肠和束缚应激诱导SD大鼠PI-IBS。分别给予水、乌鸡丸提取物(630 mg/kg)或匹维溴铵(13.5 mg/kg)灌胃。结果表明，乌鸡丸能有效改善腹痛、结肠动力异常和内脏高敏感性。粪便微生物区系分析显示，乌鸡丸能逆转肠道微生物区系丰富度下降的趋势，显著增加肠道菌群中阿克曼杆菌、类杆菌和副杆菌的相对丰度，而乳杆菌和普氏杆菌在PI-IBS大鼠中的相对丰度显著降低。此外，乌鸡丸通过增加粘蛋白的释放，上调紧密连接蛋白occludin和ZO-1的分布，下调MLCK在结肠上皮细胞中的表达，促进结肠黏膜杯状细胞的增殖。这些结果提示，无极丸可能通过调节肠道微生物区系和稳定肠道黏膜屏障来缓解IBS，表明使用具有益生作用的经典中药方剂可能是治疗PI-IBS的一种有前景的策略。
Emerging evidence suggests that gut microbiota contribute to the treatment of post-inflammatory irritable bowel syndrome (PI-IBS). Our previous studies have demonstrated that a Chinese formula, Wuji Wan, has the ability to mitigate abdominal pain and diarrhea in PI-IBS rats. However, little is known about the underlying mechanism and whether the gut microbiota mediate the effect of Wuji Wan on PI-IBS. Thus, the aim of this study was to determine whether Wuji Wan mitigated PI-IBS by modifying the gut microbiota. PI-IBS was induced in Sprague-Dawley rats by enema using 4% acetic acid and restraint stress. Rats were fed water, Wuji Wan extract (630 mg/kg) or pinaverium bromide (13.5 mg/kg). Our data showed that Wuji Wan effectively ameliorated abdominal pain, colonic motility abnormality and visceral hypersensitivity. Analysis of the fecal microbiota showed that Wuji Wan could reverse the reduction in richness of the gut microbiota and significantly increase the relative abundances of Akkermansia, Bacteroides, and Parasutterella; however, Lactobacillus and Prevotella were markedly decreased in the PI-IBS rats. Moreover, Wuji Wan promoted goblet cell proliferation in the colonic mucosa by increasing the release of mucin, up-regulating the distribution of tight junction proteins Occludin and ZO-1 and down-regulating the expression of MLCK in colonic epithelial cells. These findings suggest that Wuji Wan may remit IBS by modulating the gut microbiota and stabilizing the gut mucosal barrier, indicating that the use of a classical formula of Traditional Chinese Medicine (TCM) that exhibits a prebiotic effect may be a promising strategy for PI-IBS treatment.
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影响因子：
3.6
作者：
Acosta A;Camilleri M;Shin A;Linker Nord S;O'Neill J;Gray AV;Lueke AJ;Donato LJ;Burton DD;Szarka LA;Zinsmeister AR;Golden PL;Fodor A
通讯作者：
Fodor A
影响因子：
6.3
作者：
Han, Kyungsun;Wang, Jinghwa;Kim, Hojun
通讯作者：
Kim, Hojun
影响因子：
4.3
作者：
Lee, Kang Nyeong;Lee, Oh Young
通讯作者：
Lee, Oh Young
影响因子：
3.6
作者：
Kleessen, B;Blaut, M
通讯作者：
Blaut, M
影响因子：
24.5
作者：
Jalanka-Tuovinen, Jonna;Salojarvi, Jarkko;de Vos, Willem M.
通讯作者：
de Vos, Willem M.