Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomography.
Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomography.
复制标题
DOI：
10.26508/lsa.202302409
复制
发表时间：
2024-03
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
冷冻电子断层扫描和subtomography平均揭示了新的结构特征的基础上的感光纤毛的视网膜杆，包括基体，Y-轴丝和膜之间的联系，和睫状项链的过渡区。初级纤毛在多种生物体和细胞类型中介导感觉信号，但具有适合特定角色的结构。它们的结构缺陷会导致人类的破坏性疾病，称为纤毛病。其功能的关键是其底部的结构：基体，过渡区，“Y形链接”和“睫状项链”。我们已经使用冷冻电子断层扫描与subtomogram平均和传统的透射电子显微镜，以阐明与基底区域的“连接纤毛”的杆外段在小鼠视网膜的结构。微管（MT）结构和连接它们的管腔支架复合物的纵向变化已经确定，以及膜相关的过渡区结构：Y形连接MT到膜，纤毛珠连接到它们，从细胞表面突出，形成项链状结构。这些结果代表了一个更清晰的结构支架上的分子识别的遗传学，蛋白质组学和超分辨率荧光可以放置在我们新兴的感光器感觉纤毛模型。
Cryo-electron tomography and subtomogram averaging reveal new structural features at the base of the light-sensing cilia of retinal rods, including the basal body, the Y-links between axoneme and membrane, and the ciliary necklace of the transition zone. Primary cilia mediate sensory signaling in multiple organisms and cell types but have structures adapted for specific roles. Structural defects in them lead to devastating diseases known as ciliopathies in humans. Key to their functions are structures at their base: the basal body, the transition zone, the “Y-shaped links,” and the “ciliary necklace.” We have used cryo-electron tomography with subtomogram averaging and conventional transmission electron microscopy to elucidate the structures associated with the basal region of the “connecting cilia” of rod outer segments in mouse retina. The longitudinal variations in microtubule (MT) structures and the lumenal scaffold complexes connecting them have been determined, as well as membrane-associated transition zone structures: Y-shaped links connecting MT to the membrane, and ciliary beads connected to them that protrude from the cell surface and form a necklace-like structure. These results represent a clearer structural scaffold onto which molecules identified by genetics, proteomics, and superresolution fluorescence can be placed in our emerging model of photoreceptor sensory cilia.
登录
查看更多内容
影响因子：
3.4
作者：
Danielsson, Frida;Mahdessian, Diana;Lundberg, Emma
通讯作者：
Lundberg, Emma
影响因子：
7.7
作者：
Greenan GA;Keszthelyi B;Vale RD;Agard DA
通讯作者：
Agard DA
影响因子：
--
作者：
Fry AM;Sampson J;Shak C;Shackleton S
通讯作者：
Shackleton S
影响因子：
7.8
作者：
Anderson, R G
通讯作者：
Anderson, R G
影响因子：
64.5
作者：
Gilliam JC;Chang JT;Sandoval IM;Zhang Y;Li T;Pittler SJ;Chiu W;Wensel TG
通讯作者：
Wensel TG