Antiserum from mice vaccinated with modified vaccinia Ankara virus expressing African horse sickness virus (AHSV) VP2 provides protection when it is administered 48h before, or 48h after challenge.
Antiserum from mice vaccinated with modified vaccinia Ankara virus expressing African horse sickness virus (AHSV) VP2 provides protection when it is administered 48h before, or 48h after challenge.
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DOI：
10.1016/j.antiviral.2015.01.009
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发表时间：
2015-04
影响因子：
7.6
通讯作者：
Castillo-Olivares J
中科院分区：
文献类型：
--
作者：
Calvo-Pinilla E;de la Poza F;Gubbins S;Mertens PP;Ortego J;Castillo-Olivares J
使用供体的脾细胞和抗血清在IFNAR −/−小鼠中进行被动免疫研究。用表达非洲马瘟病毒（AHSV）VP 2的修饰的安卡拉牛痘（MVA）免疫供体。AHSV攻击后，脾细胞受体对临床体征的保护较差，对病毒血症没有保护。AHSV攻击后，抗血清受体对病毒血症和临床体征具有高度保护作用。MVA-VP 2疫苗免疫与中和抗体密切相关，表明血清治疗的潜力。先前的研究表明，表达AHSV血清型4的VP 2的重组改良安卡拉牛痘（MVA）病毒（MVA-VP 2）在马中诱导病毒中和抗体，并保护干扰素α受体基因敲除小鼠（IFNAR −/−）免受攻击。后续实验表明，被动转移抗血清，从MVA-VP 2免疫供体受体小鼠1小时前的挑战，赋予完全的临床保护和显着减少病毒血症。这些研究已扩展到确定MVA-VP 2疫苗诱导的抗血清在攻毒前48小时或攻毒后48小时给药时的保护作用。此外，进行脾细胞的被动转移以评估它们是否赋予免疫学上未处理的受体小鼠任何程度的免疫力。因此，从已经用MVA-VP 2或野生型MVA（MVA-wt）接种的小鼠组收集抗血清和脾细胞，用于受体小鼠的被动免疫。后者随后用AHSV-4（以及适当的接种或未接种对照动物）攻毒，并通过比较给药组和对照组之间的临床体征、致死率和病毒血症来评估保护作用。与对照组相比，所有抗血清受体均表现出较高的疾病保护（即使在攻毒后48小时免疫的小鼠中，存活率也为100%），并且病毒血症在统计学上显著减少。与接受来自MVA-wt疫苗接种的脾细胞的小鼠相比，接受来自MVA-VP 2疫苗接种的脾细胞的小鼠组仅显示出40%的存活率，病毒血症略有减少。这些结果证实了MVA-VP 2疫苗接种赋予的保护性免疫的主要体液性质，并显示了施用MVA-VP 2特异性抗血清作为AHSV紧急治疗的潜力。
Passive immunisation studies were conducted in IFNAR −/− mice using splenocytes and antiserum from donors. Donors were immunised with modified vaccinia Ankara (MVA) expressing African horse sickness virus (AHSV) VP2. After AHSV challenge, splenocyte recipients were poorly protected against clinical signs and not protected against viraemia. After AHSV challenge, antiserum recipients were highly protected against viraemia and clinical signs. MVA-VP2 vaccination immunity is strongly associated with neutralising antibodies, indicating potential for sero-therapy. Previous studies show that a recombinant modified vaccinia Ankara (MVA) virus expressing VP2 of AHSV serotype 4 (MVA-VP2) induced virus neutralising antibodies in horses and protected interferon alpha receptor gene knock-out mice (IFNAR −/−) against challenge. Follow up experiments indicated that passive transfer of antiserum, from MVA-VP2 immune donors to recipient mice 1 h before challenge, conferred complete clinical protection and significantly reduced viraemia. These studies have been extended to determine the protective effect of MVA-VP2 vaccine-induced antiserum, when administered 48 h before, or 48 h after challenge. In addition, passive transfer of splenocytes was undertaken to assess if they confer any degree of immunity to immunologically naïve recipient mice. Thus, antisera and splenocytes were collected from groups of mice that had been vaccinated with MVA-VP2, or wild type MVA (MVA-wt), for passive immunisation of recipient mice. The latter were subsequently challenged with AHSV-4 (together with appropriate vaccinated or unvaccinated control animals) and protection was assessed by comparing clinical signs, lethality and viraemia between treated and control groups. All antiserum recipients showed high protection against disease (100% survival rates even in mice that were immunised 48 h after challenge) and statistically significant reduction or viraemia in comparison with the control groups. The mouse group receiving splenocytes from MVA-VP2 vaccinates, showed only a 40% survival rate, with a small reduction in viraemia, compared to those mice that had received splenocytes from MVA-wt vaccinates. These results confirm the primarily humoral nature of protective immunity conferred by MVA-VP2 vaccination and show the potential of administering MVA-VP2 specific antiserum as an emergency treatment for AHSV.
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影响因子：
5.5
作者：
Alberca, Berta;Bachanek-Bankowska, Katarzyna;Cabana, Marta;Calvo-Pinilla, Eva;Viaplana, Elisenda;Frost, Lorraine;Gubbins, Simon;Urniza, Alicia;Mertens, Peter;Castillo-Olivares, Javier
通讯作者：
Castillo-Olivares, Javier
影响因子：
5.5
作者：
Draper SJ;Cottingham MG;Gilbert SC
通讯作者：
Gilbert SC
影响因子：
3.7
作者：
BURRAGE, TG;TREVEJO, R;LAEGREID, WW
通讯作者：
LAEGREID, WW
影响因子：
5.4
作者：
Garcia-Arriaza, Juan;Cepeda, Victoria;Esteban, Mariano
通讯作者：
Esteban, Mariano
影响因子：
1.8
作者：
El Garch, H.;Crafford, J. E.;Minke, J. M.
通讯作者：
Minke, J. M.