A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions.
A multiplexed assay to detect antimicrobial peptides in biological fluids and cell secretions.
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DOI：
10.1016/j.jim.2013.09.001
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发表时间：
2013-11-29
影响因子：
2.2
通讯作者：
Ackerman, Margaret E.
中科院分区：
文献类型：
--
作者：
Boesch, Austin W.;Zhao, Yifeng;Landman, Alison S.;Garcia, Marta Rodriguez;Fahey, John V.;Wira, Charles R.;Ackerman, Margaret E.
粘膜组织是防御潜在病原体的前线，粘膜提供保护的一种方式是通过分泌抗菌素来干扰潜在病原体，并招募和改变免疫细胞的反应。在这里，我们描述了适应微球格式的ELISA法，促进了抗菌肽的同时定量，包括Elafin、MIP3RANTES、HBD2、HBD3、SLPI、RANTES、SDF1、乳铁蛋白、LL-37和HNP1-3。该多重检测方法具有良好的重复性，大多数分析物在两个数量级的浓度范围内呈线性关系，与宫颈阴道灌洗液等生物液兼容，与传统的ELISA法相比，显著节省了成本和样本。
Mucosal tissues represent the front line in defense against potential pathogens, and one means by which mucosa provide protection is via the secretion of antimicrobials which can interfere with potential pathogens as well as recruit and modify the responses of immune cells. Here we describe adaptation of ELISA assays to microsphere format, facilitating simultaneous quantification of antimicrobial peptides including elafin, MIP3α, HBD2, HBD3, SLPI, RANTES, SDF1, lactoferrin, LL-37, and HNP1-3. The multiplexed assay exhibits excellent reproducibility, shows linearity over a two order of magnitude concentration range for most analytes, is compatible with biological fluids such as cervicovaginal lavage fluid, and presents significant cost and sample savings relative to traditional ELISA assays.
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影响因子：
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影响因子：
2.2
作者：
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通讯作者：
Ackerman, Margaret E.
影响因子：
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