Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques.
Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques.
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DOI：
10.3791/60965
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发表时间：
2020-05-07
期刊：
影响因子：
--
通讯作者：
Morton GJ
中科院分区：
文献类型：
--
作者：
Faber CL;Matsen ME;Meek TH;Krull JE;Morton GJ
Stereotactic surgery is an essential tool in the modern neuroscience lab. However, being able to precisely and accurately target difficult to reach brain regions still represents a challenge, particularly when targeting brain structures along the midline. These challenges include avoiding the superior sagittal sinus and third ventricle and being able to consistently target selective, discrete brain nuclei. In addition, more advanced neuroscience techniques (e.g., optogenetics, fiber photometry, and 2-photon imaging) rely on targeted implantation of significant hardware to the brain and spatial limitations represent a common hindrance. Here we present a modifiable protocol for stereotactic targeting of rodent brain structures using an angled coronal approach that may be adapted for 1) either mouse or rat; 2) various neuroscience techniques and 3) for multiple brain regions. As a representative example, we include calculation of stereotactic coordinates for targeting the mouse hypothalamic ventromedial nucleus (VMN) for an optogenetic inhibition experiment. This procedure begins with the bilateral microinjection of an adeno-associated virus (AAV) encoding a light-sensitive chloride channel (SwiChR++) to a cre-dependent mouse model, followed by the angled bilateral implantation of fiberoptic cannulae. Using this approach, our findings show that activation of a subset of VMN neurons is required for intact glucose counterregulatory responses to insulin-induced hypoglycemia. Here we describe an adaptable stereotactic procedure that can be utilized for targeting challenging and difficult to reach brain regions, due to spatial limitations, using an angled coronal approach. This protocol is adaptable to both mouse and rat and can be applied to diverse neuroscientific applications including implantation of cannulas and microinjections of viral constructs for fiber photometry and/or chemogenetic and optogenetic studies.
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影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
影响因子：
7.7
作者：
Faber CL;Matsen ME;Velasco KR;Damian V;Phan BA;Adam D;Therattil A;Schwartz MW;Morton GJ
通讯作者：
Morton GJ
影响因子：
1.2
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通讯作者：
McClung, Colleen A.
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1.2
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通讯作者：
Mao, Yingwei
影响因子：
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作者：
Roth BL
通讯作者：
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