Assessment of human papillomavirus in lung tumor tissue.
Assessment of human papillomavirus in lung tumor tissue.
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DOI：
10.1093/jnci/djr003
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发表时间：
2011-03-16
期刊：
影响因子：
--
通讯作者：
Caporaso NE
中科院分区：
文献类型：
--
作者：
Koshiol J;Rotunno M;Gillison ML;Van Doorn LJ;Chaturvedi AK;Tarantini L;Song H;Quint WG;Struijk L;Goldstein AM;Hildesheim A;Taylor PR;Wacholder S;Bertazzi PA;Landi MT;Caporaso NE
全世界每年有超过100万人死于肺癌。虽然已经确定了几种人乳头瘤病毒（HPV）相关的癌症，但HPV在肺癌发生中的作用仍然存在争议。我们从意大利基于人群的病例对照研究中选择了450名肺癌患者，环境和遗传学在肺癌病因学中。这些患者是从那些有足够数量的未染色组织切片的患者中挑选出来的，包括所有从不吸烟的患者和随机抽取的剩余患者样本。我们使用实时聚合酶链反应（PCR）检测这些患者的HPV DNA，特别是HPV16的E6基因序列和HPV18的E7基因序列。我们还对来自所有不吸烟者和随机选择的吸烟者的92个样本进行了pcr检测，以检测至少54种粘膜HPV基因型。在严格的PCR清洁条件下，从乙醇或福尔马林固定石蜡包埋的肿瘤组织中提取DNA。研究了HPV在肿瘤组织中的流行情况。450名患者中有399名的样本具有足够的DNA进行分析。大多数患者为当前吸烟者（220例，占48.9%），92例患者为女性（20.4%）。当使用HPV16和HPV18型特异性引物时，两个标本在低拷贝数下HPV16阳性，但在其他类型特异性HPV16检测中呈阴性。无论是这些样本，还是其他接受广泛HPV类型检查的样本，都没有对任何一种HPV类型呈阳性。当避免DNA污染并使用最先进的高灵敏度HPV DNA检测分析时，我们发现没有证据表明HPV与具有代表性的西方人群的肺癌相关。我们的结果提供了迄今为止最有力的证据来排除HPV在西方人群肺癌发生中的作用。
Lung cancer kills more than 1 million people worldwide each year. Whereas several human papillomavirus (HPV)–associated cancers have been identified, the role of HPV in lung carcinogenesis remains controversial. We selected 450 lung cancer patients from an Italian population–based case–control study, the Environment and Genetics in Lung Cancer Etiology. These patients were selected from those with an adequate number of unstained tissue sections and included all those who had never smoked and a random sample of the remaining patients. We used real-time polymerase chain reaction (PCR) to test specimens from these patients for HPV DNA, specifically for E6 gene sequences from HPV16 and E7 gene sequences from HPV18. We also tested a subset of 92 specimens from all never-smokers and a random selection of smokers for additional HPV types by a PCR-based test for at least 54 mucosal HPV genotypes. DNA was extracted from ethanol- or formalin-fixed paraffin-embedded tumor tissue under strict PCR clean conditions. The prevalence of HPV in tumor tissue was investigated. Specimens from 399 of 450 patients had adequate DNA for analysis. Most patients were current (220 patients or 48.9%) smokers, and 92 patients (20.4%) were women. When HPV16 and HPV18 type–specific primers were used, two specimens were positive for HPV16 at low copy number but were negative on additional type-specific HPV16 testing. Neither these specimens nor the others examined for a broad range of HPV types were positive for any HPV type. When DNA contamination was avoided and state-of-the-art highly sensitive HPV DNA detection assays were used, we found no evidence that HPV was associated with lung cancer in a representative Western population. Our results provide the strongest evidence to date to rule out a role for HPV in lung carcinogenesis in Western populations.
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影响因子：
3.1
作者：
Gravitt, PE;Peyton, C;Shah, KV
通讯作者：
Shah, KV
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通讯作者：
Heard, Isabelle