High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus.
High-Throughput Sequencing Reveals Circulating miRNAs as Potential Biomarkers of Kidney Damage in Patients with Systemic Lupus Erythematosus.
复制标题
高通量测序揭示循环 miRNA 是系统性红斑狼疮患者肾脏损伤的潜在生物标志物。
DOI：
10.1371/journal.pone.0166202
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Aroca G
中科院分区：
文献类型：
--
作者：
Navarro-Quiroz E;Pacheco-Lugo L;Lorenzi H;Díaz-Olmos Y;Almendrales L;Rico E;Navarro R;España-Puccini P;Iglesias A;Egea E;Aroca G
肾脏受累是系统性红斑狼疮（SLE）最严重的表现之一。肾活检是了解患者是否患有狼疮性肾炎以及肾脏疾病程度的金标准。然而，活检有各种并发症，出血是最常见的。因此，开发SLE患者肾脏疾病的替代性非侵入性诊断测试是当务之急。微小RNA（miRNAs）在各种组织中差异表达，并且其表达的变化与几种病理过程相关。本研究的目的是确定狼疮性肾炎患者血浆样本中miRNAs丰度的变化，这可能有助于诊断SLE患者的肾损害。这是一项观察性病例对照横断面研究，其中我们将不同程度狼疮患者与无肾脏受累的SLE患者和健康对照个体进行比较，以表征miRNA的差异丰度谱。我们发现89个miRNAs在狼疮肾炎患者和健康对照组之间的丰度发生了变化，17个miRNAs在有或无肾脏受累的SLE患者之间显示出显著变化。对一组miRNAs进行qPCR验证，结果显示，5种miRNAs的平均检测灵敏度为97%，特异性为70.3%，阳性预测值为82.5%，阴性预测值为96%，诊断效率为87.9%。这些结果强烈提示miR-221- 5 p、miR-380- 3 p、miR-556- 5 p、miR-758- 3 p和miR-3074- 3 p是SLE患者狼疮性肾炎的潜在诊断生物标志物。所观察到的miRNA丰度差异模式可能在SLE肾损害的病理生理学中具有功能意义。
Renal involvement is one of the most severe manifestations of systemic lupus erythematosus (SLE). Renal biopsy is the gold standard when it comes to knowing whether a patient has lupus nephritis, and the degree of renal disease present. However, the biopsy has various complications, bleeding being the most common. Therefore, the development of alternative, non-invasive diagnostic tests for kidney disease in patients with SLE is a priority. Micro RNAs (miRNAs) are differentially expressed in various tissues, and changes in their expression have been associated with several pathological processes. The aim of this study was to identify changes in the abundance of miRNAs in plasma samples from patients with lupus nephritis that could potentially allow the diagnosis of renal damage in SLE patients. This is an observational case-control cross-sectional study, in which we characterized the differential abundance profiles of miRNAs among patients with different degrees of lupus compared with SLE patients without renal involvement and healthy control individuals. We found 89 miRNAs with changes in their abundance between lupus nephritis patients and healthy controls, and 17 miRNAs that showed significant variations between SLE patients with or without renal involvement. Validation for qPCR of a group of miRNAs on additional samples from lupus patients with or without nephritis, and from healthy individuals, showed that five miRNAs presented an average detection sensitivity of 97%, a specificity of 70.3%, a positive predictive value of 82.5%, a negative predictive value of 96% and a diagnosis efficiency of 87.9%. These results strongly suggest that miR-221-5p, miR-380-3p, miR-556-5p, miR-758-3p and miR-3074-3p are potential diagnostic biomarkers of lupus nephritis in patients with SLE. The observed differential pattern of miRNA abundance may have functional implications in the pathophysiology of SLE renal damage.
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影响因子：
2.6
作者：
Dai, Y.;Huang, Y-S;Yin, Y-B
通讯作者：
Yin, Y-B
DOI：
10.1073/pnas.0902745106
发表时间：
2009-04-28
影响因子：
11.1
作者：
Ben-Sasson, Shlomo Z.;Hu-Li, Jane;Paul, William E.
通讯作者：
Paul, William E.
影响因子：
4
作者：
Dai, Yong;Sui, Weiguo;Huang, YuanShuai
通讯作者：
Huang, YuanShuai
影响因子：
--
作者：
Mok, CC;Tang, SSK;Petri, M
通讯作者：
Petri, M
影响因子：
5.9
作者：
Mok, CC;Tang, SSK
通讯作者：
Tang, SSK