Effects of Bacillus amyloliquefaciens FZB42 on lettuce growth and health under pathogen pressure and its impact on the rhizosphere bacterial community.
Effects of Bacillus amyloliquefaciens FZB42 on lettuce growth and health under pathogen pressure and its impact on the rhizosphere bacterial community.
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DOI：
10.1371/journal.pone.0068818
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Grosch R
中科院分区：
文献类型：
--
作者：
Chowdhury SP;Dietel K;Rändler M;Schmid M;Junge H;Borriss R;Hartmann A;Grosch R
土传病原体立枯丝核菌（Rhizoctoniasolani）是造成世界范围内多种重要作物损失的原因。缺乏有效的控制策略和对有机食品日益增长的需求刺激了生物控制的研究。本研究的目的是评估商业化的接种剂解淀粉芽孢杆菌FZB 42对生菜生长和健康的根际竞争力，以及其对土著根际细菌群落的影响，在田间和盆栽试验。两个实验的结果表明，FZB 42能够在田间莴苣生长期内有效地定殖根际（7.45至6.61 Log 10 CFU g-1根干质量）。通过在种植前和种植后用FZB 42处理生菜幼苗，生菜底腐病的严重程度（DS）从DS 5类的严重症状显著降低到DS 3类的轻微症状表现。基于16 S rRNA基因的指纹分析方法末端限制性片段长度多态性（T-RFLP）表明，与FZB 42的治疗没有土著根际细菌群落的重大影响。然而，细菌群落显示出明显的时间转移。结果还表明，病原菌R.接种后根际微生物群落结构发生变化。因此，我们揭示了接种剂FZB 42可以在根际中成功地建立自己，而不会对根际细菌群落表现出任何持久的影响。
The soil-borne pathogen Rhizoctonia solani is responsible for crop losses on a wide range of important crops worldwide. The lack of effective control strategies and the increasing demand for organically grown food has stimulated research on biological control. The aim of the present study was to evaluate the rhizosphere competence of the commercially available inoculant Bacillus amyloliquefaciens FZB42 on lettuce growth and health together with its impact on the indigenous rhizosphere bacterial community in field and pot experiments. Results of both experiments demonstrated that FZB42 is able to effectively colonize the rhizosphere (7.45 to 6.61 Log 10 CFU g−1 root dry mass) within the growth period of lettuce in the field. The disease severity (DS) of bottom rot on lettuce was significantly reduced from severe symptoms with DS category 5 to slight symptom expression with DS category 3 on average through treatment of young plants with FZB42 before and after planting. The 16S rRNA gene based fingerprinting method terminal restriction fragment length polymorphism (T-RFLP) showed that the treatment with FZB42 did not have a major impact on the indigenous rhizosphere bacterial community. However, the bacterial community showed a clear temporal shift. The results also indicated that the pathogen R. solani AG1-IB affects the rhizosphere microbial community after inoculation. Thus, we revealed that the inoculant FZB42 could establish itself successfully in the rhizosphere without showing any durable effect on the rhizosphere bacterial community.
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影响因子：
--
作者：
Grosch, Rita;Scherwinski, Katja;Berg, Gabriele
通讯作者：
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影响因子：
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影响因子：
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通讯作者：
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4.2
作者：
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通讯作者：
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