The transcriptome profile of the mosquito Culex quinquefasciatus following permethrin selection.
The transcriptome profile of the mosquito Culex quinquefasciatus following permethrin selection.
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DOI：
10.1371/journal.pone.0047163
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Liu N
中科院分区：
文献类型：
--
作者：
Reid WR;Zhang L;Liu F;Liu N
为了深入了解致倦库蚊抗药性形成过程中的基因相互作用和复杂的调控系统，我们对二氯苯醚菊酯筛选后的致病库蚊进行了全转录组分析。比较了低抗性亲本品系HAmCqG0及其氯菊酯选择高抗后代HAmCqG8的基因表达谱，发现HAmCqG8中分别有367个和3982个基因表达上调和下调，表明氯氰菊酯选择参与了多个基因的响应。然而，在选择氯菊酯后的HAmCqG8蚊子中，发现上调和下调的基因之间存在类似的总体累积基因表达丰度，这表明通过平衡上调和下调基因来对杀虫剂做出动态平衡反应。结构和/或表皮结构功能是仅有的两个丰富的GO下调基因项，而上调基因的丰富GO项主要出现在催化和代谢功能中，它们代表三种功能类别：电子载体活性、结合和催化活性。有趣的是，这三个功能类别中的功能性GO术语在P450和蛋白酶/丝氨酸蛋白酶中被压倒性地过度表达。P450在抗药性形成中的重要作用已经得到了广泛的研究，但对蛋白酶/丝氨酸蛋白酶在抗性中的作用了解较少。因此，对这些蛋白的功能进行表征，包括它们的消化、催化和蛋白酶活性；信号转导和蛋白运输、免疫和储存的调节；以及它们在蚊子抗药性形成中的确切功能，将为了解基因如何在抗性中相互联系和调节提供新的见解。
To gain valuable insights into the gene interaction and the complex regulation system involved in the development of insecticide resistance in mosquitoes Culex quinquefasciatus, we conducted a whole transcriptome analysis of Culex mosquitoes following permethrin selection. Gene expression profiles for the lower resistant parental mosquito strain HAmCqG0 and their permethrin-selected high resistant offspring HAmCqG8 were compared and a total of 367 and 3982 genes were found to be up- and down-regulated, respectively, in HAmCqG8, indicating that multiple genes are involved in response to permethrin selection. However, a similar overall cumulative gene expression abundance was identified between up- and down-regulated genes in HAmCqG8 mosquitoes following permethrin selection, suggesting a homeostatic response to insecticides through a balancing of the up- and down-regulation of the genes. While structural and/or cuticular structural functions were the only two enriched GO terms for down-regulated genes, the enriched GO terms obtained for the up-regulated genes occurred primarily among the catalytic and metabolic functions where they represented three functional categories: electron carrier activity, binding, and catalytic activity. Interestingly, the functional GO terms in these three functional categories were overwhelmingly overrepresented in P450s and proteases/serine proteases. The important role played by P450s in the development of insecticide resistance has been extensively studied but the function of proteases/serine proteases in resistance is less well understood. Hence, the characterization of the functions of these proteins, including their digestive, catalytic and proteinase activities; regulation of signaling transduction and protein trafficking, immunity and storage; and their precise function in the development of insecticide resistance in mosquitoes will provide new insights into how genes are interconnected and regulated in resistance.
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