Iron uptake from transferrin and asialotransferrin by hepatocytes from chronically alcohol-fed rats.
Iron uptake from transferrin and asialotransferrin by hepatocytes from chronically alcohol-fed rats.
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长期饮酒大鼠的肝细胞从转铁蛋白和脱唾液酸转铁蛋白中摄取铁。
DOI：
10.1111/j.1530-0277.1992.tb00684.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Beloqui,O
中科院分区：
文献类型：
--
作者：
Potter,BJ;McHugh,TA;Beloqui,O
长期酒精摄入通常与铁稳态的改变和碳水化合物缺乏型转铁蛋白血清水平的增加有关。由于肝脏是一个主要的铁储存网站，也合成转铁蛋白，正常的血清铁转运蛋白，本研究的目的是测试的假设，这些干扰铁稳态所造成的改变肝细胞铁摄取异常转铁蛋白。为了实现这一点，我们研究了饲喂De Carli和Lieber酒精饮食的雄性Sprague-道利大鼠肝细胞从转铁蛋白和脱唾液酸转铁蛋白中的铁摄取。铁的摄取从转铁蛋白的肝细胞从酒精大鼠是小于60%的控制值，并在50 mM乙醇的存在下，进一步降低到35%的相应的控制细胞的摄取。与正常转铁蛋白相比，两组大鼠去唾液酸转铁蛋白的铁摄取均减少;对照细胞减少13%，酒精喂养大鼠肝细胞减少39%。然而，酒精对来自酒精喂养大鼠或其配对喂养对照的肝细胞的无唾液酸转铁蛋白摄取没有进一步影响。与肝细胞结合的转铁蛋白也受到酒精饮食的影响。虽然在37°C下结合没有差异，但来自酒精喂养大鼠的细胞在4°C下结合了85%的总结合率，而对照肝细胞为44%。无唾液酸转铁蛋白的肝细胞结合也获得了类似的值;酒精喂养导致4°C下的结合从对照细胞的58%增加到73%。培养基中酒精的存在并没有显著影响任何这些值。这些研究表明，慢性酒精摄入量减少肝脏铁从转铁蛋白的吸收。在一些酗酒者中观察到的铁沉着症可能不是由从无唾液酸转铁蛋白中摄取铁引起的，因为至少在大鼠中，所有组中肝细胞从该分子中摄取铁都减少。这里提供的数据表明，转铁蛋白的膜受体再循环机制存在缺陷，这是长期酗酒的结果。
Chronic alcohol intake is often associated with alterations to iron homeostasis and an increase in the serum levels of carbohydrate‐deficient transferrin. As the liver is a major iron storage site and also synthesizes transferrin, the normal serum iron transport protein, the aim of this study was to test the hypothesis that these disturbances in iron homeostasis were caused by altered hepatocyte iron uptake from the abnormal transferrin. To achieve this, we have investigated iron uptake from both transferrin and asialotransferrin by hepatocytes from male Sprague‐Dawley rats fed the De Carli and Lieber alcohol diet. Iron uptake from transferrin by hepatocytes from alcoholic rats was less than 60% that of control values, and in the presence of 50 mM ethanol decreased still further to 35% of the uptake by the corresponding control cells. Iron uptake from rat asialotransferrin was reduced in both groups when compared to that observed from normal transferrin; 13% by control cells and 39% by hepatocytes from alcohol‐fed rats. Alcohol, however, had no further effect on asialotransferrin uptake by either hepatocytes from alcohol‐fed rats, or their pair‐fed controls. Transferrin binding to hepatocytes was also influenced by the alcohol diet. Although there was no difference in binding at 37°C, cells from alcohol‐fed rats bound 85% of this total at 4°C, compared to 44% by control hepatocytes. Similar values were also obtained for hepatocyte binding of asialotransferrin; alcohol feeding resulted in an increase in binding at 4°C to 73% from 58% with control cells. The presence of alcohol in the medium did not significantly affect any of these values. These studies suggest that chronic alcohol intake decreases liver iron uptake from transferrin. The siderosis seen in some alcoholics may not be caused by iron uptake from asialotransferrin since, at least in the rat, hepatocytic iron uptake from this molecule is decreased in all groups. The data presented here suggest that there is a defect in the membrane receptor recycling mechanism for transferrin, which occurs as a result of long‐term alcohol abuse.
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使用色谱聚焦检测酒精受试者血清中的转铁蛋白变异体。
影响因子：
9.3
作者：
E. Storey;U. Mack;L. Powell;J. Halliday
通讯作者：
J. Halliday
在乙醇存在下，分离的肝细胞对转铁蛋白的铁吸收抑制是乙醇代谢的 pH 依赖性结果。
DOI：
10.1111/j.1530-0277.1986.tb05125.x
发表时间：
1986
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Beloqui,O;Nunes,RM;Blades,B;Berk,PD;Potter,BJ
通讯作者：
Potter,BJ
转铁蛋白聚糖：酗酒和肝铁质沉着症之间可能存在的联系。
DOI：
10.1111/j.1530-0277.1984.tb05513.x
发表时间：
1984
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
E. Regoeczi;P. Chindemi;M. Debanne
通讯作者：
M. Debanne
酗酒者体内的铁储藏室。
影响因子：
--
作者：
O. Lundvall;A. Weinfeld;P. Lundin
通讯作者：
P. Lundin
乙醇对大鼠肝蛋白质合成和分泌的急性影响。
影响因子：
29.4
作者：
E. Baraona;P. Pikkarainen;M. Salaspuro;Frances Finkelman;C. Lieber
通讯作者：
C. Lieber