Time of day and genotype sensitivity adjust molecular responses to temperature stress in sorghum
Time of day and genotype sensitivity adjust molecular responses to temperature stress in sorghum
复制标题
一天中的时间和基因型敏感性调节高粱对温度胁迫的分子反应
DOI：
10.1111/tpj.16467
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Nagel, Dawn H.
中科院分区：
文献类型：
--
作者：
Bonnot, Titouan;Somayanda, Impa;Jagadish, S. V. Krishna;Nagel, Dawn H.
高粱是被认为对极端环境具有耐受性的四种主要C4作物之一。高粱对温度胁迫表现出不同的生长响应，这取决于遗传背景的敏感性。大约一半的高粱转录本表现出昼夜节律表达，强调与环境的显著协调。然而，分子动力学如何促进一天中特定时间背景下基因型特异性应激反应的理解尚不清楚。我们研究了温度胁迫和日照时间是否会影响热敏感型和耐热型高粱基因型的基因表达动态。我们发现，一天中的时间高度影响温度胁迫反应，这可以用大多数热反应基因的节律性表达来解释。这种效应在耐热基因型中更为明显，这表明一天中的时间和/或生物钟对基因表达的调节更强。基因型差异主要表现在平均基因表达水平上，这可能是耐寒和敏感高粱品种对温度胁迫敏感性差异的原因。我们还确定了温度胁迫在一天中的时间和基因型特异性方式下改变的基因组。这些包括转录调节因子和Ca2+结合EF - hand蛋白家族的几个成员。我们假设这些基因在不同基因型之间的表达差异以及与时间无关的调节可能有助于热反应途径的基因型特异性微调。这些发现提供了一个新的机会，可以根据特定基因对温度胁迫的时间和基因型变异反应，选择性地针对特定基因开发具有气候适应性的作物。
Sorghum is one of the four major C4 crops that are considered to be tolerant to environmental extremes. Sorghum shows distinct growth responses to temperature stress depending on the sensitivity of the genetic background. About half of the transcripts in sorghum exhibit diurnal rhythmic expressions emphasizing significant coordination with the environment. However, an understanding of how molecular dynamics contribute to genotype‐specific stress responses in the context of the time of day is not known. We examined whether temperature stress and the time of day impact the gene expression dynamics in thermo‐sensitive and thermo‐tolerant sorghum genotypes. We found that time of day is highly influencing the temperature stress responses, which can be explained by the rhythmic expression of most thermo‐responsive genes. This effect is more pronounced in thermo‐tolerant genotypes, suggesting a stronger regulation of gene expression by the time of day and/or by the circadian clock. Genotypic differences were mostly observed on average gene expression levels, which may be responsible for contrasting sensitivities to temperature stress in tolerant versus susceptible sorghum varieties. We also identified groups of genes altered by temperature stress in a time‐of‐day and genotype‐specific manner. These include transcriptional regulators and several members of the Ca2+‐binding EF‐hand protein family. We hypothesize that expression variation of these genes between genotypes along with time‐of‐day independent regulation may contribute to genotype‐specific fine‐tuning of thermo‐responsive pathways. These findings offer a new opportunity to selectively target specific genes in efforts to develop climate‐resilient crops based on their time‐of‐day and genotype variation responses to temperature stress.
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DOI：
10.1093/plcell/koab205
发表时间：
2021-11-04
期刊：
The Plant cell
影响因子：
--
作者：
Pardo J;VanBuren R
通讯作者：
VanBuren R
影响因子：
3.5
作者：
A. Vennapusa;Y. Assefa;David Sebela;Impa M. Somayanda;R. Perumal;D. Riechers;P. V. Vara Prasad;S. Jagadish
通讯作者：
A. Vennapusa;Y. Assefa;David Sebela;Impa M. Somayanda;R. Perumal;D. Riechers;P. V. Vara Prasad;S. Jagadish
细胞质游离钙的昼夜节律振荡调节拟南芥生物钟
DOI：
10.17863/cam.27571
发表时间：
2018
期刊：
--
影响因子：
--
作者：
Marti Ruiz M
通讯作者：
Marti Ruiz M
影响因子：
4.6
作者：
Blair, Emily J.;Bonnot, Titouan;Nagel, Dawn H.
通讯作者：
Nagel, Dawn H.
DOI：
10.1111/j.1550-7408.1958.tb02555.x
发表时间：
1958-01-01
期刊：
JOURNAL OF PROTOZOOLOGY
影响因子：
--
作者：
SWEENEY, BM;HASTINGS, JW
通讯作者：
HASTINGS, JW