Serum levels of miR-21-5p and miR-339-5p associate with occupational trichloroethylene hypersensitivity syndrome.
Serum levels of miR-21-5p and miR-339-5p associate with occupational trichloroethylene hypersensitivity syndrome.
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miR-21-5p 和 miR-339-5p 的血清水平与职业性三氯乙烯超敏综合征相关。
DOI：
10.1186/s12995-021-00308-0
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发表时间：
2021-05-17
期刊：
影响因子：
--
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Liu W;Zheng J;Ren X;Xie Y;Lin D;Li P;Lv Y;Hoi MPM;Zhang Y;Liu J
三氯乙烯（TCE）超敏反应综合征（THS）是一种剂量依赖性和潜在的危及生命的疾病。在这项研究中，我们试图识别THS相关的miRNA并评估其潜在的临床价值。使用5名患者和5名匹配的TCE接触者的血清样品筛选差异miRNA。另外34名患者和34名匹配的TCE接触者用于使用SYBR™绿色PCR和MGB PCR验证显著差异的miRNA。通过支持向量机（SVM）算法建立基于这些miRNAs的诊断模型。通过斯皮尔曼相关性检验分析差异miRNA与肝功能之间的相关性。共发现69个miRNAs差异表达。miR-21- 5 p和miR-339- 5 p被证实在患者中具有显著更高的表达。疾病模型的敏感性、特异性和准确性分别为100%、75%和86%。这两种miRNAs与肝功能显著相关。这些结果表明，THS患者血清中的miRNAs谱发生了显著变化，miR-21- 5 p和miR-339- 5 p与THS相关。在线版本包含补充材料，可通过10.1186/s12995-021-00308-0获得。
Trichloroethylene (TCE) hypersensitivity syndrome (THS) is a dose-independent and potentially life-threatening disease. In this study, we sought to identify THS-related miRNAs and evaluate its potential clinical value. Serum samples of five patients and five matched TCE contacts were used for screening differential miRNAs. Another 34 patients and 34 matched TCE contacts were used for verifying significantly differential miRNAs with SYBR™ Green PCR and MGB PCR. The diagnostic model based on these miRNAs was established via the support vector machine (SVM) algorithm. Correlation between differential miRNAs and liver function was analyzed via the Spearman correlation test. A total of 69 miRNAs was found to be differentially expressed. MiR-21-5p and miR-339-5p were verified to have significant higher expressions in patients. The sensitivity, specificity and accuracy of disease model were 100, 75 and 86%, respectively. The two miRNAs showed significant correlations with liver function. These findings suggested that miRNAs profiles in serum of THS patients had changed significantly, and miR-21-5p and miR-339-5p were associated with THS. The online version contains supplementary material available at 10.1186/s12995-021-00308-0.
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影响因子：
10.4
作者：
Pastino GM;Yap WY;Carroquino M
通讯作者：
Carroquino M
影响因子：
3.6
作者：
Mainhagu, J.;Morrison, C.;Brusseau, M. L.
通讯作者：
Brusseau, M. L.
DOI：
10.5539/gjhs.v7n1p161
发表时间：
2014-08-22
期刊：
Global journal of health science
影响因子：
--
作者：
Singthong S;Pakkong P;Choosang K;Wongsanit S
通讯作者：
Wongsanit S
影响因子：
--
作者：
Friesen, Melissa C.;Locke, Sarah J.;Vermeulen, Roel
通讯作者：
Vermeulen, Roel
影响因子：
3.1
作者：
Watanabe, Hideaki
通讯作者：
Watanabe, Hideaki