Recall of B cell memory depends on relative locations of prime and boost immunization.
Recall of B cell memory depends on relative locations of prime and boost immunization.
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DOI：
10.1126/sciimmunol.abn5311
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发表时间：
2022-05-06
影响因子：
24.8
通讯作者：
Kelsoe, Garnett
中科院分区：
文献类型：
--
作者：
Kuraoka, Masayuki;Yeh, Chen-Hao;Bajic, Goran;Kotaki, Ryutaro;Song, Shengli;Windsor, Ian;Harrison, Stephen C.;Kelsoe, Garnett
免疫或微生物感染不仅可以在全身而且可以在局部建立长期的B细胞记忆。有证据表明，局部B细胞记忆有助于二次激发后的早期局部浆细胞反应，然而，尚不清楚局部性是否在记忆B细胞参与回忆记忆中心（GC）中发挥任何作用，这对于更新其B细胞抗原受体（BCR）至关重要。使用单个B细胞培养和命运作图，我们已经表征了在引发的局部或远端部位加强免疫后回忆GC中的BCR库。用同源抗原进行的局部加强比远端加强更有效地募集原代GC B细胞的后代以回忆GC。回忆通过局部加强诱导的GC含有显著更多的免疫球蛋白（IG）突变水平升高的B细胞和更高的亲合力BCR。这种局部偏好不受阻断CD 40：CD 154相互作用以终止活跃的GC应答的影响。用异源抗原进行局部加强免疫可诱导具有富集了与初免和加强免疫抗原交叉反应性的B细胞群的继发性GC;相反，远端加强免疫后交叉反应性GC B细胞很少。我们的研究结果表明，本地B细胞的记忆是保留在记忆B细胞，GC B细胞和GC-表型B细胞，是独立的组织GC结构，这些持久的“启动B细胞”有助于回忆GC反应在本地网站。我们的研究结果表明，在体液免疫的重要性，并告知不断演变的病毒的系列疫苗接种策略。记忆B细胞在回忆中枢中的参与取决于增强是在启动部位的局部还是远端。
Immunization or microbial infection can establish long-term B cell memory not only systemically but also locally. Evidence has suggested that local B cell memory contributes to early local plasmacytic responses after secondary challenge, however; it is unclear whether locality plays any role in participation of memory B cells in recall germinal centers (GCs) which is essential for updating their B cell antigen receptors (BCRs). Using single B cell culture and fate-mapping, we have characterized BCR repertoires in recall GCs following boost immunizations at sites local or distal to the priming. Local boosts with homologous antigen recruit the progeny of primary GC B cells to recall GCs more efficiently than do distal boosts. Recall GCs elicited by local boosts contain significantly more B cells with elevated levels of Immunoglobulin (Ig) mutation and higher avidity BCRs. This local preference is unaffected by blocking CD40:CD154 interaction to terminate active, GC responses. Local boosts with heterologous antigens elicit secondary GCs with B cell populations enriched for cross-reactivity to the prime and boost antigens; in contrast, cross-reactive GC B cells are rare following distal boosts. Our results suggest that local B cell memory is retained in the form of memory B cells, GC B cells and GC-phenotype B cells that are independent of organized GC structures and that these persistent “primed B cells” contribute to recall GC responses at local sites. Our findings indicate the importance of locality in humoral immunity and inform serial vaccination strategies for evolving viruses. The participation of memory B cells in recall germinal centers depends on whether the boost is local or distal to the priming site.
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影响因子：
--
作者：
Kepler TB
通讯作者：
Kepler TB
影响因子：
46.9
作者：
通讯作者：
--
影响因子：
30.5
作者：
Dogan, Ismail;Bertocci, Barbara;Weill, Jean-Claude
通讯作者：
Weill, Jean-Claude
DOI：
10.1073/pnas.1102571108
发表时间：
2011-07-12
影响因子：
11.1
作者：
Kuraoka, Masayuki;Holl, T. Matt;Kelsoe, Garnett
通讯作者：
Kelsoe, Garnett
影响因子：
30.3
作者：
Haynes BF;Shaw GM;Korber B;Kelsoe G;Sodroski J;Hahn BH;Borrow P;McMichael AJ
通讯作者：
McMichael AJ