Osteogenic growth peptide-loaded 3D-printed PCL scaffolds for the promotion of osteogenesis through the ERK pathway
Osteogenic growth peptide-loaded 3D-printed PCL scaffolds for the promotion of osteogenesis through the ERK pathway
复制标题
负载成骨生长肽的 3D 打印 PCL 支架通过 ERK 途径促进成骨
DOI：
10.1016/j.matdes.2020.108811
复制
发表时间：
2020-08
影响因子：
8.4
通讯作者：
Jinsong Pan
中科院分区：
文献类型：
--
作者：
Qing Wang;Xiaojie Yang;Guangfei Wang;Leilei Wan;Shiwei Wang;Xiaoyong Niu;Jiannan Wu;Jinsong Pan
本研究旨在通过成骨生长肽（OGP）修饰的聚己内酯（PCL）支架改善体外成骨分化和体内骨形成。利用计算机辅助设计（CAD）软件设计三维（3D）打印的PCL支架，并通过3D逐层熔融沉积制备。通过表面胺化对支架表面进行修饰后，利用静电自组装方法将OGP成功地负载到支架上。体外研究这些支架材料对大鼠骨髓间充质干细胞（BMSCs）生物相容性及成骨分化的影响。这种修饰不影响细胞的粘附和增殖，但在体外具有明显的成骨作用，这可能与激活丝裂原活化蛋白激酶/细胞外调节蛋白激酶（MAPK/ERK）信号通路有关。基于植入大鼠颅骨缺损模型后获得的结果，PCL/OGP支架显著促进骨形成和加速矿化。本研究所采用的新的改性方法，将进一步提高PCL支架在组织工程中的应用。
This study aims to improve the osteogenic differentiationin vitroand bone formationin vivothrough polycaprolactone (PCL) scaffolds modified with the osteogenic growth peptide (OGP). Three-dimensional (3D)-printed PCL scaffolds were designed with computer-aided design (CAD) software and fabricated by 3D layer-by-layer fused deposition. After the scaffold surfaces were modified by surface amination, the OGP was successfully loaded onto the scaffolds using a electrostatic self-assembly method. The effects of these scaffolds on the biocompatibility and osteogenic differentiation of rat bone marrow-derived stem cells (BMSCs) were studiedin vitro. The modification did not influence the adhesion or proliferation, but had a notable osteogenic effectin vitro.This may be attributed to the activation of the mitogen-activated protein kinase/extracellular regulated protein kinase (MAPK/ERK) signalling pathway. Based on the results obtained after implantation into a rat cranial defect model, the PCL/OGP scaffolds notably promoted bone formation and accelerated mineralisation. The innovative modification method introduced in this work, will improve the application of PCL scaffolds in tissue engineering.
登录
查看更多内容
影响因子：
2.7
作者：
Bruyas A;Lou F;Stahl AM;Gardner M;Maloney W;Goodman S;Yang YP
通讯作者：
Yang YP
用于促进骨再生的血管化 3D 打印支架。
影响因子：
14
作者：
Yan, Yufei;Chen, Hao;Deng, Lianfu
通讯作者：
Deng, Lianfu
DOI：
10.1002/jbm.a.36234
发表时间：
2018-01
期刊：
Journal of biomedical materials research. Part A
影响因子：
--
作者：
Dey RE;Wimpenny I;Gough JE;Watts DC;Budd PM
通讯作者：
Budd PM
DOI：
10.1016/j.msec.2016.11.076
发表时间：
2017-04
期刊：
Materials science & engineering. C, Materials for biological applications
影响因子：
--
作者：
Saleheh Shahmoradi;F. Yazdian;F. Tabandeh;Z. Soheili;Ashraf Sadat Hatamian Zarami;M. Navaei-Nigjeh
通讯作者：
Saleheh Shahmoradi;F. Yazdian;F. Tabandeh;Z. Soheili;Ashraf Sadat Hatamian Zarami;M. Navaei-Nigjeh
DOI：
10.1016/j.msec.2017.11.017
发表时间：
2018-03
期刊：
Materials science & engineering. C, Materials for biological applications
影响因子：
--
作者：
Rezvan Mobasseri;Lingling Tian;M. Soleimani;S. Ramakrishna;H. Naderi-manesh
通讯作者：
Rezvan Mobasseri;Lingling Tian;M. Soleimani;S. Ramakrishna;H. Naderi-manesh