The effects of hypoxia and temperature on metabolic aspects of embryonic development in the annual killifish Austrofundulus limnaeus.
The effects of hypoxia and temperature on metabolic aspects of embryonic development in the annual killifish Austrofundulus limnaeus.
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DOI：
10.1007/s00360-014-0803-6
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Podrabsky JE
中科院分区：
文献类型：
--
作者：
Anderson SN;Podrabsky JE
澳洲石蜥的胚胎在耐受长时间完全缺氧的能力方面是例外的。低氧和缺氧是它们发育环境的正常组成部分。在这里，我们将胚胎暴露于两种不同温度（25 ℃和30 ℃）下的一系列PO2水平，以研究氧气减少和温度升高对发育速率，心率和代谢酶能力的综合影响。缺氧降低了总体发育速度，并导致心率随阶段而下降。然而，在器官发生之前的早期发育速率对PO2不敏感。在高氧条件下，提高孵化温度可使发育速率增加，但在严重缺氧条件下，发育进程受阻。孵化前胚胎DNA含量与PO2呈正相关。柠檬酸合酶，乳酸脱氢酶，磷酸烯醇式丙酮酸羧激酶的能力都降低了胚胎发育在缺氧条件下。A. Limnaeus能够在广泛的PO2范围内正常发育，与大多数其他脊椎动物相反，严重缺氧不是致畸剂。A. Limnaeus不通过代谢酶乳酸脱氢酶、柠檬酸合酶或磷酸烯醇丙酮酸羧激酶的酶活性的能力的增加来响应缺氧。相反，它们似乎调整整个胚胎的代谢能力，以匹配氧气的可用性。然而，在低氧饲养的胚胎中DNA含量的减少表明，细胞的酶能力可能保持不变，在响应缺氧，而减少的能力可能表明减少细胞数量在低氧胚胎。
Embryos of Austrofundulus limnaeus are exceptional in their ability to tolerate prolonged bouts of complete anoxia. Hypoxia and anoxia are a normal part of their developmental environment. Here, we exposed embryos to a range of PO2 levels at two different temperatures (25 C and 30 C) to study the combined effects of reduced oxygen and increased temperature on developmental rate, heart rate, and metabolic enzyme capacity. Hypoxia decreased overall developmental rate and caused a stage-specific decline in heart rate. However, the rate of early development prior to the onset of organogenesis is insensitive to PO2. Increased incubation temperature caused an increase in the developmental rate at high PO2s, but hindered developmental progression under severe hypoxia. Embryonic DNA content in pre-hatching embryos was positively correlated with PO2. Citrate synthase, lactate dehydrogenase, and phosphoenolpyruvate carboxykinase capacity were all reduced in embryos developing under hypoxic conditions. Embryos of A. limnaeus are able to develop normally across a wide range of PO2s and contrary to most other vertebrates severe hypoxia is not a teratogen. Embryos of A. limnaeus do not respond to hypoxia through an increase in the capacity for enzymatic activity of the metabolic enzymes lactate dehydrogenase, citrate synthase, or phosphoenolpyruvate carboxykinase. Instead they appear to adjust whole-embryo metabolic capacity to match oxygen availability. However, decreased DNA content in hypoxia-reared embryos suggests that cellular enzymatic capacity may remain unchanged in response to hypoxia, and the reduced capacity may rather indicate reduced cell number in hypoxic embryos.
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DOI：
10.1016/s0022-0981(02)00329-5
发表时间：
2002-11-06
影响因子：
2
作者：
Cooper, RU;Clough, LM;West, TL
通讯作者：
West, TL
DOI：
10.1139/z59-069
发表时间：
1959-01-01
期刊：
CANADIAN JOUR ZOOL
影响因子：
--
作者：
GARSIDE, E. T.
通讯作者：
GARSIDE, E. T.
影响因子：
--
作者：
GRABOWSKI, C
通讯作者：
GRABOWSKI, C
影响因子：
--
作者：
DIMICHELE, L;TAYLOR, MH
通讯作者：
TAYLOR, MH
DOI：
10.1002/jez.632
发表时间：
2010-11-01
影响因子：
2.8
作者：
Chennault, T.;Podrabsky, J. E.
通讯作者：
Podrabsky, J. E.