Distinct roles for the NK cell-activating receptors in mediating interactions with dendritic cells and tumor cells.
Distinct roles for the NK cell-activating receptors in mediating interactions with dendritic cells and tumor cells.
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DOI：
10.4049/jimmunol.1002597
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Krams SM
中科院分区：
文献类型：
--
作者：
Wai LE;Garcia JA;Martinez OM;Krams SM
NK cells are innate immune cells that are important in tumor immunity, but also have the ability to modulate the adaptive immune system through cytokine production or direct cell–cell interactions. This study investigates the interaction of NK cells with dendritic cells (DCs) and tumor cells, and the role of specific NK cell-activating receptors in this process. Primary rat NK cells and an NK cell line produced IFN-γ when cocultured with either DCs or the rat hepatoma cell line McA-RH7777 (McA). This NK cell activation by DCs and McA required cell-cell contact and was dependent on distinct NK-activating receptors. Silencing NK cell expression of NKp46 and NKp30 significantly diminished DC- and McA-mediated NK cell IFN-γ production, respectively. NK cells killed immature and mature DCs independently of NKp46, NKp30, and NKG2D; however, cytotoxicity against McA cells was dependent on NKp30 and NKG2D. Thus, we have shown in this study that NKp30 plays dual activating roles in NK–McA tumor interactions by mediating cytokine production and cytotoxicity. More importantly, NK cells are activated by both DCs and hepatoma cells to produce IFN-γ, but require distinct NK cell-activating receptors, NKp46 and NKp30, respectively. Our data suggest that therapeutics could be developed specifically to target NK–DC interactions without compromising NK tumor immunity.
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DOI：
10.1084/jem.20010938
发表时间：
2002-02-04
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Gerosa F;Baldani-Guerra B;Nisii C;Marchesini V;Carra G;Trinchieri G
通讯作者：
Trinchieri G
影响因子：
10.7
作者：
Hollyoake, M;Campbell, RD;Aguado, B
通讯作者：
Aguado, B
影响因子：
32.4
作者：
Lucas, Mathias;Schachterle, William;Diefenbach, Andreas
通讯作者：
Diefenbach, Andreas
影响因子：
56.9
作者：
Bauer, S;Groh, V;Spies, T
通讯作者：
Spies, T
影响因子：
30.5
作者：
Andoniou, CE;van Dommelen, SLH;Degli-Esposti, MA
通讯作者：
Degli-Esposti, MA