Reciprocal activating interaction between natural killer cells and dendritic cells.
Reciprocal activating interaction between natural killer cells and dendritic cells.
复制标题
DOI：
10.1084/jem.20010938
复制
发表时间：
2002-02-04
期刊：
影响因子：
--
通讯作者：
Trinchieri G
中科院分区：
文献类型：
--
作者：
Gerosa F;Baldani-Guerra B;Nisii C;Marchesini V;Carra G;Trinchieri G
我们分析了人外周血自然杀伤（NK）细胞和单核细胞来源的未成熟树突状细胞（DC）之间的相互作用。新鲜的NK细胞被激活，如CD 69抗原的诱导表达所示，并且它们的细胞溶解活性通过与脂多糖（LPS）处理的成熟DC或在成熟刺激物LPS、结核分枝杆菌或干扰素（IFN）-α存在下与未成熟DC接触而强烈增强。相反，在成熟刺激物存在下与未成熟DC一起培养的新鲜NK细胞强烈增强DC成熟和白细胞介素（IL）-12产生。IL-2激活的NK细胞直接诱导DC成熟，并增强其刺激同种异体幼稚CD 4 + T细胞的能力。NK细胞的作用是细胞接触依赖性的，尽管IFN-γ和TNF的分泌也有助于DC的成熟。在外周血淋巴细胞内，与DC的相互激活相互作用仅限于NK细胞，因为其他淋巴细胞亚群既不被诱导表达CD 69，也不被诱导成熟与DC接触。这些数据首次证明了NK细胞和DC之间的双向串扰，其中由IL-2或成熟DC激活的NK细胞诱导DC成熟。
We analyzed the interaction between human peripheral blood natural killer (NK) cells and monocyte-derived immature dendritic cells (DC). Fresh NK cells were activated, as indicated by the induced expression of the CD69 antigen, and their cytolytic activity was strongly augmented by contact with lipopolysaccharide (LPS)-treated mature DC, or with immature DC in the presence of the maturation stimuli LPS, Mycobacterium tuberculosis or interferon (IFN)-α. Reciprocally, fresh NK cells cultured with immature DC in the presence of the maturation stimuli strongly enhanced DC maturation and interleukin (IL)-12 production. IL-2–activated NK cells directly induced maturation of DC and enhanced their ability to stimulate allogeneic naive CD4+ T cells. The effects of NK cells were cell contact dependent, although the secretion of IFN-γ and TNF also contributed to DC maturation. Within peripheral blood lymphocytes the reciprocal activating interaction with DC was restricted to NK cells, because the other lymphocyte subsets were neither induced to express CD69, nor induced to mature in contact with DC. These data demonstrated for the first time a bidirectional cross talk between NK cells and DC, in which NK cells activated by IL-2 or by mature DC induce DC maturation.
登录
查看更多内容
影响因子：
82.9
作者：
Fernandez, NC;Lozier, A;Zitvogel, L
通讯作者：
Zitvogel, L
影响因子：
--
作者：
Trinchieri G
通讯作者：
Trinchieri G
DOI：
10.1084/jem.185.12.2053
发表时间：
1997-06-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Carbone E;Ruggiero G;Terrazzano G;Palomba C;Manzo C;Fontana S;Spits H;Kärre K;Zappacosta S
通讯作者：
Zappacosta S
影响因子：
64.8
作者：
Ridge, JP;Di Rosa, F;Matzinger, P
通讯作者：
Matzinger, P
影响因子：
7.8
作者：
Trinchieri, G
通讯作者：
Trinchieri, G