Association analysis between SNPs in IL-28B gene and the progress of hepatitis B infection in Han Chinese.
Association analysis between SNPs in IL-28B gene and the progress of hepatitis B infection in Han Chinese.
复制标题
DOI：
10.1371/journal.pone.0050787
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Chen J;Wang L;Li Y;Cai B;Fu Y;Liao Y;Zhang J
作为一项候选基因关联研究，我们研究了IL-28 B基因中SNP与HBV感染不同结局（包括LC和HCC发生）的遗传关联。中国汉族受试者分为两组：406例由CHB引起的LC和406例由CHB引起的HCC。从全血样本中提取基因组DNA，采用高分辨率熔解曲线（HRM）法检测SNPs。PCR扩增在相同条件下在96孔板中在Real-Time PCR System中进行。以341例肝细胞癌和356例肝细胞癌患者为研究对象，采用独立样本进行验证。393例CHB患者和244例健康对照者。进展为LC或HCC的CHB患者在rs 12979860中显示出显著不同的频率（p = 0.046）。 与LC相比，HCC患者更频繁地携带rs 12979860中的T等位基因。在独立样本中发现了相同的结果。IL-28 B rs 12979860 C/T多态性T等位基因在HCC患者中的发生率高于LC患者。携带该等位基因似乎增加了发生HCC的风险。IL-28 B基因多态性可能赋予B型肝炎感染进展和程度的症状特异性。
As a candidate gene association study, we investigated the genetic association of SNPs in IL-28B genes with different outcomes of HBV infection, including LC and HCC occurrence. Chinese Han subjects were categorized into two groups: 406 LC caused by CHB and 406 HCC caused by CHB. Genomic DNA was isolated from whole blood samples, SNPs were detected using high resolution melting curve (HRM) method. PCR amplification was carried out under the same conditions in a 96-well plate in Real-Time PCR System. Then 341 LC and 356 HCC patients caused by HBV infection were analyzed as a verification by independent sample. 393 CHB patients and 244 health subjects were included as control. CHB patients who progress to LC or HCC showed a significant different frequency in rs12979860 (p = 0.046). Patients with HCC carried more frequently the T alleles in rs12979860 comparison to LC. Same results were found in the independent sample. IL-28B rs12979860 C/T polymorphism T allele appears to be more prevalent in patients with HCC than in LC. Carriage of this allele seems to enhance the risk for developing HCC. Gene polymorphism of IL-28B may confer symptomatic specificity in progress and extent of hepatitis B infection.
登录
查看更多内容
影响因子：
--
作者：
Pearlman, Brian L
通讯作者：
Pearlman, Brian L
DOI：
10.1086/657146
发表时间：
2010-12-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Martin MP;Qi Y;Goedert JJ;Hussain SK;Kirk GD;Hoots WK;Buchbinder S;Carrington M;Thio CL
通讯作者：
Thio CL
影响因子：
25.7
作者：
Fabris, Carlo;Falleti, Edmondo;Toniutto, Pierluigi
通讯作者：
Toniutto, Pierluigi
影响因子：
7.8
作者：
Migita, Kiyoshi;Sawakami-Kobayashi, Kazumi;Ishibashi, Hiromi
通讯作者：
Ishibashi, Hiromi
影响因子：
4.8
作者：
Dumoutier, L;Tounsi, A;Renauld, JC
通讯作者：
Renauld, JC