Comparative proteomic analysis of dental cementum from deciduous and permanent teeth.
Comparative proteomic analysis of dental cementum from deciduous and permanent teeth.
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DOI：
10.1111/jre.12808
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发表时间：
2021-01
影响因子：
3.5
通讯作者：
Kantovitz KR
中科院分区：
文献类型：
--
作者：
Giovani PA;Martins L;Salmon CR;Mofatto LS;Leme AFP;Puppin-Rontani RM;Kolli TN;Foster BL;Nociti FH Jr;Kantovitz KR
牙骨质是覆盖牙根的矿化组织，在牙体附着体中起重要作用。乳牙和恒牙DC的差异尚未被研究。我们假设DC基质的蛋白质组学分析可以识别乳牙骨质（DecDC）和恒牙骨质（PermDC）的成分差异，这可能反映了生理或病理差异，例如乳牙的牙根吸收是生理的，而恒牙列的牙根吸收可能是病理性的。取乳牙（n=25）和恒牙（n=12）的蛋白提取物（DecDC 5池，每池5颗牙；PermDC 4池，每池3颗牙）。样品变性，蛋白质提取，还原，烷基化，消化，并通过液相色谱质谱（LCMS/MS）分析。归一化谱计数采用β -二项统计检验，显著性水平为5%，确定差异表达蛋白。免疫组织化学用于验证所选蛋白。共鉴定出510个蛋白：123个（24.1%）为DecDC专属蛋白；PermDC独占128个（25.1%）；259个（50.8%）在DecDC和PermDC中普遍表达。在60种差异表达蛋白中，DecDC中检测到17种（28.3%），包括髓过氧化物酶（MPO），而PermDC中检测到43种（71.7%），包括decorin （DCN）和骨钙素（BGLAP）。总体而言，基因本体（Gene Ontology， GO）分析表明，所有表达的蛋白都与GO生物过程有关，包括定位和应激反应；差异表达蛋白的氧化石墨烯分子功能富集在细胞粘附、分子结合、细胞骨架蛋白结合、结构分子活性和大分子复合物结合等方面。免疫组织化学证实了人类牙齿中选择的差异表达蛋白的趋势。DecDC和PermDC的蛋白质组有明显的差异。这些发现可能会导致DecDC和PermDC之间发育差异的新见解，以及更好地理解生理/病理事件，如根吸收。
Dental cementum (DC) is a mineralized tissue covering tooth roots that plays a critical role in dental attachment. Differences in deciduous vs. permanent tooth DC have not been explored. We hypothesized that proteomic analysis of DC matrix would identify compositional differences in deciduous (DecDC) vs. permanent (PermDC) cementum that might reflect physiological or pathological differences, such as root resorption that is physiological in deciduous teeth but can be pathological in the permanent dentition. Protein extracts from deciduous (n=25) and permanent (n=12) teeth were pooled (five pools of DecDC, five teeth each; four pools of PermDC, three teeth each). Samples were denatured, and proteins were extracted, reduced, alkylated, digested, and analyzed by liquid chromatography mass spectrometry (LCMS/MS). The beta-binomial statistical test was applied to normalized spectrum counts with 5% significance level to determine differentially expressed proteins. Immunohistochemistry was used to validate selected proteins. A total of 510 proteins were identified: 123 (24.1%) exclusive to DecDC; 128 (25.1%) exclusive to PermDC; 259 (50.8%) commonly expressed in both DecDC and PermDC. Out of 60 differentially expressed proteins, 17 (28.3%) were detected in DecDC, including myeloperoxidase (MPO), whereas 43 (71.7%) were detected in PermDC, including decorin (DCN) and osteocalcin (BGLAP). Overall, Gene Ontology (GO) analysis indicated that all expressed proteins were related to GO Biological Processes that included localization and response to stress; and the GO Molecular Function of differentially expressed proteins were enriched in cell adhesion, molecular binding, cytoskeletal protein binding, structural molecular activity and macromolecular complex binding. Immunohistochemistry confirmed the trends for selected differentially expressed proteins in human teeth. Clear differences were found between the proteomes of DecDC and PermDC. These findings may lead to new insights into developmental differences between DecDC and PermDC, as well as to a better understanding of physiological/pathological events such as root resorption.
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DOI：
10.1016/j.matbio.2017.10.005
发表时间：
2019-01
期刊：
Matrix biology : journal of the International Society for Matrix Biology
影响因子：
--
作者：
Buraschi S;Neill T;Iozzo RV
通讯作者：
Iozzo RV
关于牙骨的发现。
影响因子：
3.5
作者：
Foster BL
通讯作者：
Foster BL
影响因子：
3.2
作者：
HAKKINEN, L;OKSALA, O;LARJAVA, H
通讯作者：
LARJAVA, H
影响因子：
4.2
作者：
DEFRANCO, DJ;GLOWACKI, J;LIAN, JB
通讯作者：
LIAN, JB
影响因子：
4.3
作者：
Giovani, Priscila A.;Salmon, Cristiane R.;Kantovitz, Kamila R.
通讯作者：
Kantovitz, Kamila R.