Murine CD146 is widely expressed on endothelial cells and is recognized by the monoclonal antibody ME-9F1.
Murine CD146 is widely expressed on endothelial cells and is recognized by the monoclonal antibody ME-9F1.
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DOI：
10.1007/s00418-008-0379-x
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发表时间：
2008-04
影响因子：
2.3
通讯作者：
Klugewitz, Katja
中科院分区：
文献类型：
--
作者：
Schrage, Arnhild;Loddenkemper, Christoph;Erben, Ulrike;Lauer, Uta;Hausdorf, Gert;Jungblut, Peter R.;Johnson, Judith;Knolle, Percy A.;Zeitz, Martin;Hamann, Alf;Klugewitz, Katja
内皮细胞在血液和下层组织之间的分子交换中起重要作用，但也在免疫细胞的交换中起重要作用。内皮分子S-Endo 1抗原（CD 146）优先位于内皮连接处，并被认为支持内皮完整性。在这项研究中，我们表明，单克隆抗体ME-9 F1识别的小鼠CD 146的细胞外部分。利用ME-9 F1，我们发现CD 146在所分析的小鼠组织中的内皮细胞上高度表达并广泛分布。与也在T细胞或滤泡树突状细胞上表达CD 146的人类相反，鼠CD 146尽管水平较低，但仅在NK1.1+细胞的子集上发现。抗鼠CD 146的抗体可用于免疫磁性分选不仅来自肝脏而且来自各种其他器官的原代内皮细胞。在体外，没有证据表明内皮细胞单层的形成和完整性或T细胞的跨内皮迁移受到抗体与CD 146结合或抗原交联的影响。这使得抗体ME-9 F1成为一种极好的工具，特别是用于功能研究中预期使用的鼠内皮细胞的离体分离。
The endothelium plays an important role in the exchange of molecules, but also of immune cells between blood and the underlying tissue. The endothelial molecule S-Endo 1 antigen (CD146) is preferentially located at endothelial junctions and has been claimed to support endothelial integrity. In this study we show that the monoclonal antibody ME-9F1 recognizes the extracellular portion of murine CD146. Making use of ME-9F1 we found CD146 highly expressed and widely spread on endothelial cells in the analyzed murine tissues. In contrast to humans that express CD146 also on T cells or follicular dendritic cells, murine CD146 albeit at low levels was only found on a subset of NK1.1+ cells. The antibody against murine CD146 is useful for immunomagnetic sorting of primary endothelial cells not only from the liver but from various other organs. In vitro, no evidence was seen that the formation and integrity of endothelial monolayers or the transendothelial migration of T cells was affected by antibody binding to CD146 or by crosslinking of the antigen. This makes the antibody ME-9F1 an excellent tool especially for the ex vivo isolation of murine endothelial cells intended to be used in functional studies.
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影响因子：
4.8
作者：
Hirata, K;Ishida, T;Quertermous, T
通讯作者：
Quertermous, T
DOI：
10.1006/bbrc.1996.0037
发表时间：
1996-01-05
影响因子：
3.1
作者：
Bardin, N;Frances, V;Sampol, J
通讯作者：
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影响因子：
20.3
作者：
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通讯作者：
Dignat-George, F
影响因子：
15.9
作者：
HOYER, LW;SANTOS, RPD;HOYER, JR
通讯作者：
HOYER, JR
影响因子：
3.7
作者：
HARDER, R;UHLIG, H;HAMANN, A
通讯作者：
HAMANN, A