Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus).
Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus).
复制标题
鸡两性 ESC 和 PGC 转录组测序及比较分析
DOI：
10.3390/ani10122228
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发表时间：
2020-11-27
期刊：
影响因子：
--
通讯作者：
Li B
中科院分区：
文献类型：
--
作者：
Jin K;Zhou J;Zuo Q;Song J;Zhang Y;Chang G;Chen G;Li B
鸡胚胎干细胞（ESCs）和原始生殖细胞（PGCs）的研究显示了发育生物学和转化医学的潜在应用。然而，两性胚胎干细胞和原始生殖细胞之间的区别仍然是模糊的，限制了相关研究的准确性。本研究通过分离鸡两性胚胎干细胞和两性生殖细胞，并对其进行序列测定，以探索其动态转录组。我们的研究结果为鸡两性胚胎干细胞和原始生殖细胞转录组的研究提供了基础，这将有助于其他有兴趣研究相关生物学过程的研究人员。摘要鸡多能胚胎干细胞（ESCs）和原始生殖细胞（PGCs）在发育和胚胎生物学研究中有着广泛的应用，但两性ESCs和PGCs的区别仍不清楚。本研究通过聚合酶链反应（PCR）和荧光激活细胞分选仪（FACS）鉴定特异性性别标志物来确定所采集样本的性别。利用RNA-seq研究鸡两性胚胎干细胞和生殖细胞的转录组学特征。结果表明，两性胚胎干细胞中没有显著差异表达基因（DEG），两性PGCs中有227个DEG。根据京都基因与基因组百科全书（KEGG）分析，这227个DEG主要富集在17个基因本体（GO）术语和27条通路上。此外，进行qRT-PCR以验证RNA-seq结果，结果表明Notch 1在雄性PGC中高度表达。综上所述，我们的研究结果为鸡两性胚胎干细胞和生殖细胞的研究奠定了基础，这将有助于今后相关生物学过程的研究。
Simple Summary The study of chicken embryonic stem cells (ESCs) and primordial germ cells (PGCs) showed the potential application of developmental biology and translational medicine. However, the difference between amphoteric ESCs and PGCs is still elusive, limiting the accuracy of correlative research. In this paper, chicken amphoteric ESCs and PGCs were isolated, separated, and sequenced to explore their dynamic transcriptomes. Our results provide a knowledge base of transcriptomes in chicken amphoteric ESCs and PGCs, which will help other researchers interested in studying relative biological processes. Abstract Chicken (Gallus gallus) pluripotent embryonic stem cells (ESCs) and primordial germ cells (PGCs) can be broadly applied in the research of developmental and embryonic biology, but the difference between amphoteric ESCs and PGCs is still elusive. This study determined the sex of collected samples by identifying specific sex markers via polymerase chain reaction (PCR) and fluorescence activated cell sorter (FACS). RNA-seq was utilized to investigate the transcriptomic profile of amphoteric ESCs and PGCs in chicken. The results showed no significant differentially expressed genes (DEGs) in amphoteric ESCs and 227 DEGs exhibited in amphoteric PGCs. Moreover, those 227 DEGs were mainly enriched in 17 gene ontology (GO) terms and 27 pathways according to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Furthermore, qRT-PCR was performed to verify RNA-seq results, and the results demonstrated that Notch1 was highly expressed in male PGCs. In summary, our results provided a knowledge base of chicken amphoteric ESCs and PGCs, which is helpful for future research in relevant biological processes.
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影响因子：
1.5
作者：
Disteche CM;Berletch JB
通讯作者：
Berletch JB
DOI：
10.1098/rstb.2017.0021
发表时间：
2017-11-05
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Brockdorff N
通讯作者：
Brockdorff N
影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
12.4
作者：
Haller, R.;Schwanbeck, R.;Rohwedel, J.
通讯作者：
Rohwedel, J.
影响因子：
3.8
作者：
Jung, Jin Gyoung;Lee, Young Mok;Han, Jae Yong
通讯作者：
Han, Jae Yong