Size Matters: An Evaluation of the Molecular Basis of Ontogenetic Modifications in the Composition of Bothrops jararacussu Snake Venom.
Size Matters: An Evaluation of the Molecular Basis of Ontogenetic Modifications in the Composition of Bothrops jararacussu Snake Venom.
复制标题
DOI：
10.3390/toxins12120791
复制
发表时间：
2020-12-11
期刊：
影响因子：
4.2
通讯作者：
Moura-da-Silva AM
中科院分区：
文献类型：
--
作者：
Freitas-de-Sousa LA;Nachtigall PG;Portes-Junior JA;Holding ML;Nystrom GS;Ellsworth SA;Guimarães NC;Tioyama E;Ortiz F;Silva BR;Kunz TS;Junqueira-de-Azevedo ILM;Grazziotin FG;Rokyta DR;Moura-da-Silva AM
蛇毒成分的个体发育变化已被描述，但只有少数研究试图确定靶向类似物或参与个体发育过程中基因表达改变的分子机制。在这项研究中，我们从6个不同大小的标本中解码了雅拉索巴氏杆菌毒腺转录本，并分析了19个个体的独立毒液蛋白质组组成的变异性。我们鉴定了125个不同的毒素转录本，其中73个在毒液蛋白质组中检测到，只有10个参与了个体发育变化。个体发育变异与蛇的大小呈线性相关，与繁殖期的成熟程度无关。转录组的变化高度预示着毒液蛋白质组的变化。碱性肌毒性磷脂酶A2(PLA2)是大型蛇毒中含量最丰富的成分，而小型蛇毒中的主要成分是PIII类SVMP。所鉴定的蛇毒金属蛋白酶(SVMPs)与新的序列相对应，具有较高的促凝血和出血功能。调节毒液可变性的机制主要与转录事件有关，可能包括小蛇毒液具有更高的血液毒性和更有效的捕食功能。
Ontogenetic changes in venom composition have been described in Bothrops snakes, but only a few studies have attempted to identify the targeted paralogues or the molecular mechanisms involved in modifications of gene expression during ontogeny. In this study, we decoded B. jararacussu venom gland transcripts from six specimens of varying sizes and analyzed the variability in the composition of independent venom proteomes from 19 individuals. We identified 125 distinct putative toxin transcripts, and of these, 73 were detected in venom proteomes and only 10 were involved in the ontogenetic changes. Ontogenetic variability was linearly related to snake size and did not correspond to the maturation of the reproductive stage. Changes in the transcriptome were highly predictive of changes in the venom proteome. The basic myotoxic phospholipases A2 (PLA2s) were the most abundant components in larger snakes, while in venoms from smaller snakes, PIII-class SVMPs were the major components. The snake venom metalloproteinases (SVMPs) identified corresponded to novel sequences and conferred higher pro-coagulant and hemorrhagic functions to the venom of small snakes. The mechanisms modulating venom variability are predominantly related to transcriptional events and may consist of an advantage of higher hematotoxicity and more efficient predatory function in the venom from small snakes.
登录
查看更多内容
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
3.3
作者：
Contreras Bernal, Jorge Carlos;Bisneto, Pedro Ferreira;Monteiro, Wuelton Marcelo
通讯作者：
Monteiro, Wuelton Marcelo
影响因子：
3.3
作者：
Calvete, Juan J.;Sanz, Libia;Moura-Da-Silva, Ana M.
通讯作者：
Moura-Da-Silva, Ana M.
影响因子：
2.8
作者：
Araki, S;Masuda, S;Hayashi, H
通讯作者：
Hayashi, H
影响因子：
2.8
作者：
Del-Rei, Tiago H. Moretto;Sousa, Leijiane F.;Moura-da-Silva, Ana M.
通讯作者：
Moura-da-Silva, Ana M.