Keratinocyte progenitor cells reside in human subcutaneous adipose tissue.
Keratinocyte progenitor cells reside in human subcutaneous adipose tissue.
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DOI：
10.1371/journal.pone.0118402
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ikeda S
中科院分区：
文献类型：
--
作者：
Hasegawa T;Sakamoto A;Wada A;Fukai T;Iida H;Ikeda S
脂肪源性干细胞（ASCs）向上皮细胞系的分化尚未使用标准化的方法证明。本研究调查了角质形成细胞祖细胞是否存在于ASC群体中。培养从皮下脂肪组织分离的ASCs，并通过免疫荧光显微镜和流式细胞术检测角质形成细胞祖细胞标志物p63和桥粒芯糖蛋白3（DSG 3）的表达。此外，p63和DSG 3的表达水平进行了评估之前和之后的ASC分化成脂肪细胞的实时PCR和蛋白质印迹分析，以及在皮下脂肪组织的实时逆转录聚合酶链反应。这两种标记物在ASC中表达，但在ASC分化为脂肪细胞后下调;在皮下脂肪组织中也检测到p63阳性细胞。ASCs与人成纤维细胞共培养，并与全反式维甲酸和骨形态蛋白4孵育显示DSG 3水平上调，这也增加了IV型胶原的存在。他们还表明，只有在IV型胶原蛋白的存在下，细胞角蛋白-5水平的上调。这些结果证明角质形成细胞祖细胞存在于皮下脂肪组织中。
The differentiation of adipose-derived stem cells (ASCs) towards epithelial lineages has yet to be demonstrated using a standardized method. This study investigated whether keratinocyte progenitor cells are present in the ASC population. ASCs isolated from subcutaneous adipose tissue were cultured and examined for the expression of the keratinocyte progenitor markers p63 and desmoglein 3 (DSG3) by immunofluorescence microscopy and flow cytometry. In addition, p63 and DSG3 expression levels were assessed before and after differentiation of ASCs into adipocytes by real-time PCR and western blot analysis, as well as in subcutaneous adipose tissue by real-time reverse transcriptase polymerase chain reaction. Both markers were expressed in ASCs, but were downregulated after the differentiation of ASCs into adipocytes; p63-positive cells were also detected in subcutaneous adipose tissue. ASCs co-cultured with human fibroblasts and incubated with all-trans retinoic acid and bone morphologic protein 4 showed an upregulation in DSG3 level, which was also increased in the presence of type IV collagen. They also showed an upregulation in cytokeratin-5 level only in the presence of type IV collagen. These results provide the demonstration that keratinocyte progenitor cells reside in subcutaneous adipose tissue.
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