mRNA-expression of ERα, ERβ, and PR in clonal stem cell cultures obtained from human endometrial biopsies.
mRNA-expression of ERα, ERβ, and PR in clonal stem cell cultures obtained from human endometrial biopsies.
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DOI：
10.1100/2011/949823
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发表时间：
2011
影响因子：
--
通讯作者：
Götte M
中科院分区：
文献类型：
--
作者：
Schüring AN;Braun J;Wüllner S;Kiesel L;Götte M
背景子宫内膜的增殖和分化受雌激素和孕激素的调节。子宫内膜巨大的再生能力被认为是基于成体干细胞的活性。然而，关于人子宫内膜干细胞内分泌调节机制的信息很少。在本研究中，我们研究了ERα、ERβ和PR在人子宫内膜干细胞克隆培养物中的表达，这些细胞来源于经子宫内膜活检组织。方法. 11例患者的子宫内膜组织经宫颈活检获得。将基质细胞悬浮液以克隆密度铺板并孵育15天。在一轮克隆之前和之后通过qPCR测定ERα、ERβ和PR的表达，并标准化为18 S rRNA表达。结果ERα和ERβ表达分别下调64%和89%（P = 0.002和P < 0.001）。相比之下，PR没有显着下调，由于更异质性的表达模式。结论.培养人子宫内膜间质细胞导致ERα和ERβ下调，而PR表达在我们的患者群体中保持不变。这些结果支持的假设，干细胞可能不会受到性类固醇的直接刺激，而是通过旁分泌机制内的干细胞龛。
Background. Proliferation and differentiation of the endometrium are regulated by estrogen and progesterone. The enormous regenerative capacity of the endometrium is thought to be based on the activity of adult stem cells. However, information on endocrine regulatory mechanisms in human endometrial stem cells is scarce. In the present study, we investigated the expression of ERα, ERβ, and PR in clonal cultures of human endometrial stem cells derived from transcervical biopsies. Methods. Endometrial tissue of 11 patients was obtained by transcervical biopsy. Stromal cell suspensions were plated at clonal density and incubated for 15 days. Expression of ERα, ERβ and PR was determined by qPCR prior to and after one cloning round, and normalized to 18 S rRNA expression. Results. Expression of ERα and ERβ was downregulated by 64% and 89%, respectively (P = 0.002 and P < 0.001). In contrast, PR was not significantly downregulated, due to a more heterogenous expression pattern. Conclusions. Culture of human endometrial stroma cells results in a downregulation of ERα and ERβ, while expression of PR remained unchanged in our patient collective. These results support the hypothesis that stem cells may not be subject to direct stimulation by sex steroids, but rather by paracrine mechanisms within the stem cell niche.
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影响因子：
--
作者：
Schüring AN;Braun J;Wüllner S;Kiesel L;Götte M
通讯作者：
Götte M
影响因子：
2.7
作者：
Bulun SE;Cheng YH;Pavone ME;Xue Q;Attar E;Trukhacheva E;Tokunaga H;Utsunomiya H;Yin P;Luo X;Lin Z;Imir G;Thung S;Su EJ;Kim JJ
通讯作者：
Kim JJ
影响因子：
3.6
作者：
Gargett, Caroline E.;Schwab, Kjiana E.;Wu, Di
通讯作者：
Wu, Di
影响因子：
9.8
作者：
Ikoma, Tomomi;Kyo, Satoru;Inoue, Masaki
通讯作者：
Inoue, Masaki
影响因子：
6
作者：
Tanaka, M;Kyo, S;Inoue, M
通讯作者：
Inoue, M