Target genes and structure of the direct repeats in the DNA-binding sequences of the response regulator PhoP in Streptomyces coelicolor.
Target genes and structure of the direct repeats in the DNA-binding sequences of the response regulator PhoP in Streptomyces coelicolor.
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DOI：
10.1093/nar/gkm1150
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发表时间：
2008-03
影响因子：
14.9
通讯作者：
Martin, Juan F.
中科院分区：
文献类型：
--
作者：
Sola-Landa, Alberto;Rodriguez-Garci, Antonio;Apel, Alexander Kristian;Martin, Juan F.
在链霉菌中属于pho调控基因的表达通过将响应调节因子PhoP与11-nt直接重复序列（药物）结合而受到正向调控（如野生型和ΔphoP突变体的比较所示）。这些序列已在色链霉菌的100多个基因中发现；其中20个基因克隆成功，电泳迁移率转移实验显示PhoPDBD与大多数启动子的结合。缺失实验表明，PhoPDBD的正确结合至少需要两种药物。删除1个nt，留下一个10个nt的直接重复序列，可显著降低PhoPDBD的结合。已经确定了三种不同类型的操作员。复杂算子（III类）包含多达6种药物，其中一些药物对11-nt共识序列的保守性较差，但在足迹分析中受到PhoPDBD的保护。在保守的核心药物上启动的PhoPDBD分子的协同结合似乎是几种PhoPDBD单体与这些复杂操作符结合的机制。基于信息理论的模型考虑了相邻序列对PhoPDBD结合的正或负贡献，用于推断PHO盒的结构和每个DRu的相关性。
Expression of genes belonging to the pho regulon in Streptomyces coelicolor is positively regulated (as shown by comparing the wild-type and a ΔphoP mutant) by binding of the response regulator PhoP to 11-nt direct repeats (DRus). These sequences have been found in over 100 genes of Streptomyces coelicolor; 20 of them were cloned and the binding of PhoPDBD to most of their promoters has been shown by electrophoretic mobility shift assays. Deletion experiments showed that at least two DRus are required for proper binding of PhoPDBD. Deletion of 1 nt leaving a 10-nt direct repeat reduced drastically binding of PhoPDBD. Three different types of operators have been identified. Complex operators (class III) contain up to six DRus, some of them with poor conservation of the 11-nt consensus sequence, which however were protected by PhoPDBD in footprinting analyses. A cooperative binding of PhoPDBD molecules initiated at conserved core DRus appears to be the mechanism involved in binding of several PhoPDBD monomers to those complex operators. The information theory-based model that incorporates the positive or negative contribution to the binding of PhoPDBD of adjacent sequences has been used to deduce the structure of PHO boxes and the relevance of each DRu.
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影响因子：
5.6
作者：
MAKINO, K;SHINAGAWA, H;NAKATA, A
通讯作者：
NAKATA, A
影响因子：
5.7
作者：
Blanco, AG;Sola, M;Coll, M
通讯作者：
Coll, M
影响因子：
2.8
作者：
Apel, Alexander K.;Sola-Landa, Alberto;Martin, Juan F.
通讯作者：
Martin, Juan F.
DOI：
10.1073/pnas.1934677100
发表时间：
2003-11-25
影响因子：
11.1
作者：
Challis, GL;Hopwood, DA
通讯作者：
Hopwood, DA
影响因子：
2
作者：
Schneider, TD
通讯作者：
Schneider, TD