PCR-survey of Hox-genes of the zebrafish: new sequence information and evolutionary implications.

PCR-survey of Hox-genes of the zebrafish: new sequence information and evolutionary implications.
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斑马鱼 Hox 基因的 PCR 调查:新的序列信息和进化意义。

DOI:
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发表时间:
1996
期刊:
The Journal of experimental zoology
影响因子:
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通讯作者:
G. Wagner
G. Wagner
中科院分区:
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文献类型:
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作者:
B. Misof;M. Blanco;G. Wagner

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我们使用PCR调查分析了斑马鱼的Hox基因互补序列。我们发现了18个新的斑马鱼HOM/Hox型序列和一个msh组序列。对于组1 - 3和8 - 10,我们可以明确地将斑马鱼片段分配到同源组。由于序列变异不足,同源组4 - 7的分配必须保持暂定。被分类为同源组1 - 4、8和9的成员的斑马鱼Hox片段的数量与小鼠和人基因组的相应同源组中的基因数量相同。我们发现斑马鱼和小鼠Hox基因互补之间只有两个差异:第10组中有4个推定基因(哺乳动物中有3个),第5至7组中只有7个(哺乳动物中有8个)。与先前公布的Hox基因序列的鳉鱼,大量的斑马鱼基因组10是积极的证据,在硬骨鱼之间的Hox基因互补的变化。相比之下,Hox基因互补似乎是高度保守的所有四足动物。
We analyzed the Hox gene complement of the zebrafish Danio rerio using a PCR survey. We found 18 new zebrafish HOM/Hox type sequences and one sequence of the msh group. For groups 1-3 and 8-10 we could unambiguously assign the zebrafish fragments to cognate groups. The assignment for cognate groups 4-7 had to remain tentative due to insufficient sequence variation. The number of zebrafish Hox fragments classified as members of cognate groups 1-4, 8, and 9 is identical to the number of genes in corresponding cognate groups of the mouse and human genomes. We found only two differences between the zebrafish and mouse Hox gene complement: four putative genes in group 10 (three in mammals) and only seven in the medial groups 5 to 7 (eight in mammals). Together with the previously published Hox gene sequences of the killifish, the larger number of zebrafish genes in group 10 is positive evidence for variation in the Hox gene complements among bony fish. In contrast, the Hox gene complement appears to be highly conserved among all tetrapods.
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