Primary cultures of dissociated sympathetic neurons. III. Changes in metabolism with age in culture.
Primary cultures of dissociated sympathetic neurons. III. Changes in metabolism with age in culture.
复制标题
解散的交感神经元的主要培养物。 iii。随着文化年龄的增长，新陈代谢的变化。
DOI：
10.1083/jcb.59.2.361
复制
发表时间：
1973-11
影响因子：
7.8
通讯作者：
PATTERSON, PH
中科院分区：
文献类型：
--
作者：
MAINS, RE;PATTERSON, PH
从新生大鼠的上级颈神经节分离的交感神经元的几个生化参数进行了监测，作为培养的年龄的函数。在几乎没有非神经细胞的情况下生长的神经元显示出合成和积累儿茶酚胺的能力显著增加。这种能力增加了50倍，在体外3周期间，之后，它似乎达到一个稳定的水平。最大的变化发生在第二周。这种变化的时间过程不受以较高细胞密度接种神经元的影响。在儿茶酚胺代谢的变化是远远更大的幅度和时间过程中有很大的不同，从整体的细胞生长的放射性酪氨酸纳入蛋白质，脂质合成从放射性胆碱，并纳入放射性尿苷酸沉淀的材料进行监测。在培养物所使用的总酪氨酸中，用于儿茶酚胺合成的比例在3周期间增加到25%（10倍上升）。这种变化的代谢模式在文化提出了一个成熟的过程，这可能是类似于神经元的发展在体内。
Several biochemical parameters of dissociated sympathetic neurons from superior cervical ganglia of the newborn rat were monitored as a function of age in culture. The neurons, which were grown in the virtual absence of non-neural cells, displayed a striking increase in their ability to synthesize and accumulate catecholamines. This capacity increased 50-fold during a 3-wk period in vitro, after which it appeared to reach a steady level. The major change took place during the second week. The time course of this change was not affected by plating the neurons at a higher cell density. The change in the catecholamine metabolism was far greater in magnitude and quite different in time course from the overall growth of the cells which was monitored by the incorporation of radioactive tyrosine into protein, lipid synthesis from radioactive choline, and incorporation of radioactive uridine into acid-precipitable material. Of the total tyrosine used by the cultures, the proportion devoted to catecholamine synthesis increased to 25% (a 10-fold rise) during the 3-wk period. This changing pattern of metabolism in the cultures suggested a process of maturation which may be similar to neuronal development in vivo.
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影响因子：
7.8
作者：
MAINS, RE;PATTERSON, PH
通讯作者：
PATTERSON, PH
影响因子：
2.9
作者：
THOENEN, H;SANER, A;KETTLER, R
通讯作者：
KETTLER, R
影响因子：
7.8
作者：
MAINS, RE;PATTERSON, PH
通讯作者：
PATTERSON, PH
影响因子：
2.9
作者：
THOENEN, H;SANER, A;KETTLER, R
通讯作者：
KETTLER, R
影响因子：
4.7
作者：
BLACK, IB;HENDRY, IA;IVERSEN, LL
通讯作者：
IVERSEN, LL