Parasitic modulation of host development by ubiquitin-independent protein degradation.
Parasitic modulation of host development by ubiquitin-independent protein degradation.
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DOI：
10.1016/j.cell.2021.08.029
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发表时间：
2021-09-30
期刊：
影响因子：
64.5
通讯作者：
Hogenhout SA
中科院分区：
文献类型：
--
作者：
Huang W;MacLean AM;Sugio A;Maqbool A;Busscher M;Cho ST;Kamoun S;Kuo CH;Immink RGH;Hogenhout SA
某些专性寄生虫诱导复杂的和实质性的表型变化，在其主机的方式，有利于他们的传输到其他营养水平。然而，这些变化背后的机制在很大程度上仍然未知。在这里，我们展示了SAP 05蛋白效应器从昆虫介导的植物病原植原体采取控制几个植物发育过程。这些效应因子能延长寄主的寿命，诱导植原体和载体侵染的叶片、无菌苗和器官发生丛枝状增殖。SAP 05通过介导SPL和加塔发育调节剂的同时降解而起作用，该过程依赖于劫持植物泛素受体RPN 10，而不依赖于底物泛素化。RPN 10在真核生物中高度保守，但SAP 05不结合昆虫载体RPN 10。植物RPN 10中的两个氨基酸取代产生了对SAP 05活性具有抗性的功能变体。因此，一种效应蛋白使专性寄生植原体能够在其宿主中诱导过多的发育表型。植原体SAP 05蛋白结合植物SPL和加塔转录因子，RPN 10 SAP 05以不依赖泛素的方式介导SPL和GATA的降解。SAP 05使植物发育转变并诱导丛枝病症状。植物RPN 10的工程改造赋予对SAP 05活性的抗性。介导的植物宿主的发育变化，有利于发病机制。可以调节这种效应器-受体相互作用以工程化植物抵抗寄生虫感染。
Certain obligate parasites induce complex and substantial phenotypic changes in their hosts in ways that favor their transmission to other trophic levels. However, the mechanisms underlying these changes remain largely unknown. Here we demonstrate how SAP05 protein effectors from insect-vectored plant pathogenic phytoplasmas take control of several plant developmental processes. These effectors simultaneously prolong the host lifespan and induce witches’ broom-like proliferations of leaf and sterile shoots, organs colonized by phytoplasmas and vectors. SAP05 acts by mediating the concurrent degradation of SPL and GATA developmental regulators via a process that relies on hijacking the plant ubiquitin receptor RPN10 independent of substrate ubiquitination. RPN10 is highly conserved among eukaryotes, but SAP05 does not bind insect vector RPN10. A two-amino-acid substitution within plant RPN10 generates a functional variant that is resistant to SAP05 activities. Therefore, one effector protein enables obligate parasitic phytoplasmas to induce a plethora of developmental phenotypes in their hosts. Phytoplasma SAP05 proteins bind plant SPL and GATA transcription factors and RPN10 SAP05 mediates degradation of SPLs and GATAs in a ubiquitin-independent manner SAP05 decouples plant developmental transitions and induces witches’ broom symptoms Engineering of plant RPN10 confers resistance to SAP05 activities A virulence factor from an insect-vectored parasitic phytoplasma induces ubiquitin receptor-mediated developmental changes in the plant host that favor pathogenesis. This effector-receptor interaction can be modulated to engineer plants resistant to parasitic infection.
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影响因子：
6.9
作者：
Chang SH;Tan CM;Wu CT;Lin TH;Jiang SY;Liu RC;Tsai MC;Su LW;Yang JY
通讯作者：
Yang JY
影响因子：
27.5
作者：
Huang W;Reyes-Caldas P;Mann M;Seifbarghi S;Kahn A;Almeida RPP;Béven L;Heck M;Hogenhout SA;Coaker G
通讯作者：
Coaker G
DOI：
10.1007/978-1-61779-154-3_8
发表时间：
2011-01-01
期刊：
PLANT TRANSCRIPTION FACTORS: METHODS AND PROTOCOLS
影响因子：
--
作者：
de Folter, Stefan;Immink, Richard G. H.
通讯作者：
Immink, Richard G. H.
影响因子：
14.9
作者：
Dereeper A;Guignon V;Blanc G;Audic S;Buffet S;Chevenet F;Dufayard JF;Guindon S;Lefort V;Lescot M;Claverie JM;Gascuel O
通讯作者：
Gascuel O
影响因子：
3.2
作者：
Arashida, R.;Kakizawa, S.;Namba, S.
通讯作者：
Namba, S.