Complex environmental drivers of immunity and resistance in malaria mosquitoes.
Complex environmental drivers of immunity and resistance in malaria mosquitoes.
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DOI：
10.1098/rspb.2013.2030
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发表时间：
2013-11-07
期刊：
影响因子：
--
通讯作者：
Thomas MB
中科院分区：
文献类型：
--
作者：
Murdock CC;Moller-Jacobs LL;Thomas MB
相当多的研究工作一直致力于了解蚊子先天免疫机制的遗传和分子基础。环境因素是否与这些机制相互作用以形成整体抗性在很大程度上尚未探索。在这里，我们研究如何在平均环境温度的变化，昼夜温度波动和感染的一天的时间影响斯氏按蚊感染大肠杆菌的免疫力和抵抗力。我们用定量PCR方法检测了热灭活大肠杆菌攻击后三种免疫基因的表达。杆菌我们还用活的E.大肠杆菌，并进行细菌生长测定以量化宿主抗性。平均气温、昼夜温差和感染时间直接影响蚊虫的免疫参数和抗性。此外，在不同的环境条件下，有一套复杂的双向和三向相互作用产生特异性表型变异。结果表明，蚊子的免疫力和抵抗力受到环境变量复杂相互作用的强烈影响，这对在标准实验室条件下进行的许多蚊子免疫研究的解释提出了挑战。
Considerable research effort has been directed at understanding the genetic and molecular basis of mosquito innate immune mechanisms. Whether environmental factors interact with these mechanisms to shape overall resistance remains largely unexplored. Here, we examine how changes in mean ambient temperature, diurnal temperature fluctuation and time of day of infection affected the immunity and resistance of Anopheles stephensi to infection with Escherichia coli. We used quantitative PCR to estimate the gene expression of three immune genes in response to challenge with heat-killed E. coli. We also infected mosquitoes with live E. coli and ran bacterial growth assays to quantify host resistance. Both mosquito immune parameters and resistance were directly affected by mean temperature, diurnal temperature fluctuation and time of day of infection. Furthermore, there was a suite of complex two- and three-way interactions yielding idiosyncratic phenotypic variation under different environmental conditions. The results demonstrate mosquito immunity and resistance to be strongly influenced by a complex interplay of environmental variables, challenging the interpretation of the very many mosquito immune studies conducted under standard laboratory conditions.
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影响因子：
15.1
作者：
Adamo, S. A.
通讯作者：
Adamo, S. A.
影响因子：
2.6
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通讯作者：
Christensen, BM
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Hillyer, Julian F.;Estevez-Lao, Tania Y.
通讯作者：
Estevez-Lao, Tania Y.
影响因子：
1.9
作者：
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通讯作者：
Curtis, CF
影响因子：
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作者：
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通讯作者：
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