Acacia Fiber Protects the Gut from Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Colonization Enabled by Antibiotics.
Acacia Fiber Protects the Gut from Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli Colonization Enabled by Antibiotics.
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作者：
鉴于抗生素耐药性的持续上升和新抗生素的稀缺发现，需要新的方法来对抗抗生素耐药性。在这一过程中，人们对定植动力学和内在植物性食物特性的作用知之甚少。我们试图确定植物纤维是否可以改变肠道中耐抗生素细菌的定植动态。研究人员发现，小鼠摄入抗生素显著增强了一种致病性广谱β -内酰胺酶产生的人源大肠杆菌JJ1886 （ST131-H30Rx）的肠道定植。此外，在抗生素暴露前后摄入可溶性金合欢纤维可显著减少致病性大肠杆菌的定植。16S rRNA分析和离体共培养表明，纤维作为一种益生元，诱导天然肠道大肠杆菌通过大肠杆菌素m抑制致病性大肠杆菌，从而保护肠道微生物群。纤维可能是一种有用的益生元，用于施用抗生素，以保护人类和牲畜肠道微生物群免受抗生素耐药致病菌的定植。一种基于健康的策略——人类健康和动物健康与环境相互关联的概念——对于确定从食物到临床的抗生素耐药性驱动因素是必要的。此外，人类可以从食物中摄入耐抗生素细菌，并且在发生机会性肠外感染之前很久就会无症状地或“悄无声息地”在肠道中携带这些细菌。在这里，我们确定富含纤维的食物，特别是金合欢纤维，可能是一种新的、有前途的、廉价的益生元，可以与抗生素一起使用，以保护哺乳动物（即人类和牲畜）的肠道免受耐抗生素致病菌的侵袭。
Novel approaches to combating antibiotic resistance are needed given the ever-continuing rise of antibiotic resistance and the scarce discovery of new antibiotics. Little is known about the colonization dynamics and the role of intrinsic plant-food characteristics in this process. We sought to determine whether plant fiber could alter colonization dynamics by antibiotic-resistant bacteria in the gut. We determined that ingestion of antibiotics in mice markedly enhanced gut colonization by a pathogenic extended-spectrum beta-lactamase-producing Escherichia coli strain of human origin, E. coli JJ1886 (ST131-H30Rx). Furthermore, ingestion of soluble acacia fiber before and after antibiotic exposure significantly reduced pathogenic E. coli colonization. 16S rRNA analysis and ex vivo cocultures demonstrated that fiber protected the microbiome by serving as a prebiotic, which induced native gut E. coli to inhibit pathogenic E. coli via colicin M. Fiber may be a useful prebiotic with which to administer antibiotics to protect human and livestock gut microbiomes against colonization from antibiotic-resistant, pathogenic bacteria. IMPORTANCE A One Health-based strategy—the concept that human health and animal health are interconnected with the environment—is necessary to determine the drivers of antibiotic resistance from food to the clinic. Moreover, humans can ingest antibiotic-resistant bacteria on food and asymptomatically, or “silently,” carry such bacteria in the gut long before they develop an opportunistic extraintestinal infection. Here, we determined that fiber-rich foods, in particular acacia fiber, may be a new, promising, and inexpensive prebiotic to administer with antibiotics to protect the mammalian (i.e., human and livestock) gut against such colonization by antibiotic-resistant, pathogenic bacteria.
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影响因子：
3.7
作者：
Hart ML;Meyer A;Johnson PJ;Ericsson AC
通讯作者：
Ericsson AC
DOI：
10.1128/genomea.00988-13
发表时间：
2013-11-01
期刊：
GENOME ANNOUNCEMENTS
影响因子：
--
作者：
Andersen, Paal S.;Stegger, Marc;Price, Lance B.
通讯作者：
Price, Lance B.
影响因子：
2.2
作者：
Chen, CY;Nace, GW;Irwin, PL
通讯作者：
Irwin, PL
影响因子：
5.2
作者：
Li, Qingyun;Peng, Xiyu;Patience, John F.
通讯作者：
Patience, John F.
影响因子：
6.4
作者：
Liu CM;Stegger M;Aziz M;Johnson TJ;Waits K;Nordstrom L;Gauld L;Weaver B;Rolland D;Statham S;Horwinski J;Sariya S;Davis GS;Sokurenko E;Keim P;Johnson JR;Price LB
通讯作者：
Price LB