Insights into fungal communities in composts revealed by 454-pyrosequencing: implications for human health and safety.
Insights into fungal communities in composts revealed by 454-pyrosequencing: implications for human health and safety.
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DOI：
10.3389/fmicb.2013.00164
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发表时间：
2013
影响因子：
5.2
通讯作者：
Hickey WJ
中科院分区：
文献类型：
--
作者：
De Gannes V;Eudoxie G;Hickey WJ
木质纤维素废物堆肥中的真菌群落组成进行了评估，通过454焦磷酸测序的ITS 1库来自三个主要堆肥阶段。子囊菌门代表了27，987个真菌序列中的大多数（93%）。共鉴定出102属、120种和222个操作分类单位（OTU; >97%相似性）。30个属占主导地位（约。94%的序列），在物种水平上，匹配Chaetomium funicola和Fusarium oxysporum的序列是最丰富的（分别为26%和12%）。在所有堆肥中，成熟阶段的真菌多样性超过了嗜温阶段，但在嗜热阶段发生的多样性变化没有一致的模式。确定了15种人类病原体，其中8种以前未在堆肥中确定。这项研究表明，深度测序可以阐明堆肥中的真菌群落多样性，并且这些信息可以对堆肥使用和人类健康产生重要影响。
Fungal community composition in composts of lignocellulosic wastes was assessed via 454-pyrosequencing of ITS1 libraries derived from the three major composting phases. Ascomycota represented most (93%) of the 27,987 fungal sequences. A total of 102 genera, 120 species, and 222 operational taxonomic units (OTUs; >97% similarity) were identified. Thirty genera predominated (ca. 94% of the sequences), and at the species level, sequences matching Chaetomium funicola and Fusarium oxysporum were the most abundant (26 and 12%, respectively). In all composts, fungal diversity in the mature phase exceeded that of the mesophilic phase, but there was no consistent pattern in diversity changes occurring in the thermophilic phase. Fifteen species of human pathogens were identified, eight of which have not been previously identified in composts. This study demonstrated that deep sequencing can elucidate fungal community diversity in composts, and that this information can have important implications for compost use and human health.
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影响因子：
11.8
作者：
Denning, David W.;Park, Steven;Perlin, David S.
通讯作者：
Perlin, David S.
DOI：
10.1164/ajrccm.155.4.9105079
发表时间：
1997-04-01
影响因子：
24.7
作者：
Halonen, M;Stern, DA;Martinez, FD
通讯作者：
Martinez, FD
影响因子：
4.2
作者：
Hansgate, AM;Schloss, PD;Walker, LP
通讯作者：
Walker, LP
影响因子：
2.9
作者：
Ben-Ami, Ronen;Lamaris, Gregory A.;Kontoyiannis, Dimitrios P.
通讯作者：
Kontoyiannis, Dimitrios P.
影响因子：
9.4
作者：
Buee, M.;Reich, M.;Martin, F.
通讯作者：
Martin, F.