Comparing Caenorhabditis elegans gentle and harsh touch response behavior using a multiplexed hydraulic microfluidic device.
Comparing Caenorhabditis elegans gentle and harsh touch response behavior using a multiplexed hydraulic microfluidic device.
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DOI：
10.1039/c7ib00120g
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发表时间：
2017-10-16
期刊：
影响因子：
--
通讯作者：
Fang-Yen C
中科院分区：
文献类型：
--
作者：
McClanahan PD;Xu JH;Fang-Yen C
秀丽线虫是理解触摸遗传学和生理学的重要模式系统。传统的线虫触觉检测方法，需要用探头手动触摸动物并观察其反应，但由于其低通量和定性，受到限制。我们开发了一种微流控装置，在该装置中，数十只动物受到幅度可变的空间局部化机械刺激。该装置包括供蠕虫爬行的正弦通道，以及向蠕虫传递触摸刺激的液压阀。我们使用这项测试来表征对温和触摸刺激和研究较少的刺激性(伤害性)触摸刺激的行为反应。首先，我们测量了温和和刺激性触摸的相对反应阈值。接下来，我们量化了野生型蠕虫和具有不起作用的后部触摸感受器神经元的突变体之间的感受野差异。我们发现，在轻柔的触摸下，前部触摸感受器神经元的感受野延伸到身体的后半部分。最后，我们发现，对温和触摸的行为反应并不取决于刺激前动物的运动，而是取决于之前触摸的位置。另一方面，对刺激性触摸的反应既不取决于之前的速度，也不取决于刺激的位置。温和和刺激性触摸反应特征的不同可能反映了不同机械感觉细胞的不同神经支配。我们的测试将促进机械感觉、感觉适应和伤害性感觉的研究。
The roundworm Caenorhabditis elegans is an important model system for understanding the genetics and physiology of touch. Classical assays for C. elegans touch, which involve manually touching the animal with a probe and observing its response, are limited by their low throughput and qualitative nature. We developed a microfluidic device in which several dozen animals are subject to spatially localized mechanical stimuli with variable amplitude. The device contains 64 sinusoidal channels through which worms crawl, and hydraulic valves that deliver touch stimuli to the worms. We used this assay to characterize the behavioral responses to gentle touch stimuli and the less well studied harsh (nociceptive) touch stimuli. First, we measured the relative response thresholds of gentle and harsh touch. Next, we quantified differences in the receptive fields between wild type worms and a mutant with non-functioning posterior touch receptor neurons. We showed that under gentle touch the receptive field of the anterior touch receptor neurons extends into the posterior half of the body. Finally, we found that the behavioral response to gentle touch does not depend on the locomotion of the animal immediately prior to the stimulus, but does depend on the location of the previous touch. Responses to harsh touch, on the other hand, did not depend on either previous velocity or stimulus location. Differences in gentle and harsh touch response characteristics may reflect the different innervation of the respective mechanosensory cells. Our assay will facilitate studies of mechanosensation, sensory adaptation, and nociception.
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影响因子：
6.1
作者：
Hulme, S. Elizabeth;Shevkoplyas, Sergey S.;Whitesides, George M.
通讯作者：
Whitesides, George M.
影响因子：
2.7
作者：
CHALFIE, M;SULSTON, J
通讯作者：
SULSTON, J
影响因子：
3.5
作者：
Albeg, Adi;Smith, Cody J.;Chatzigeorgiou, Marios;Feitelson, Dror G.;Hall, David H.;Schafer, William R.;Miller, David M., III;Treinin, Millet
通讯作者：
Treinin, Millet
DOI：
10.1073/pnas.1219965110
发表时间：
2013-03-19
影响因子：
11.1
作者：
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通讯作者：
Dalnoki-Veress, Kari
影响因子：
2.8
作者：
Khanafer, Khalil;Duprey, Ambroise;Berguer, Ramon
通讯作者：
Berguer, Ramon