pH-tunable oxidase-like activity of cerium oxide nanoparticles achieving sensitive fluorigenic detection of cancer biomarkers at neutral pH.
pH-tunable oxidase-like activity of cerium oxide nanoparticles achieving sensitive fluorigenic detection of cancer biomarkers at neutral pH.
复制标题
DOI：
10.1021/ac102826k
复制
发表时间：
2011-04-01
影响因子：
7.4
通讯作者：
Perez, J. Manuel
中科院分区：
文献类型：
--
作者：
Asati, Atul;Kaittanis, Charalambos;Santra, Santimukul;Perez, J. Manuel
癌症特异性生物标志物的可靠且灵敏的检测对于癌症的诊断和治疗非常重要。因此，这些生物标志物的检测必须在不同的环境中可靠且快速地进行。 ELISA 传统生物标志物筛选方法的局限性是使用不稳定成分，例如过氧化氢和辣根过氧化物酶。此前，我们报道了纳米陶瓷能够在酸性pH下氧化各种比色染料，例如TMB和AzBTS，并设计了一种用于筛选叶酸受体的测定方法。在此，我们证明纳米氧化酶氧化底物的能力可以通过调节 pH 值来调节。结果表明，纳米陶瓷可以氧化非荧光底物 ampliflu，在 pH 7.0 时氧化为非常稳定的荧光产物试卤灵，或在 pH 4.0 时氧化为非荧光刃天青。基于这些发现，我们结合蛋白 G 将抗体固定在纳米陶瓷表面，以便检测原型癌症生物标志物在 pH 7.0 下的表达，例如叶酸受体和 EpCAM。我们发现，3小时内，nanoceria分别在肺癌和乳腺癌细胞上识别出叶酸受体和EpCAM的表达。传统ELISA的读出时间为15小时，检测阈值较高，同时需要多次洗涤步骤。考虑到这些结果以及 Nanoceria 在中性 pH 值下将 ampliflu 氧化为其稳定荧光产物的能力，预计携带抗体的 Nanoceria 在实验室和即时分子诊断中的使用。
The reliable and sensitive detection of cancer-specific biomarkers is important for the diagnosis and treatment of cancer. Hence, detection of these biomarkers has to be reliably and rapidly performed in diverse settings. A limitation of the conventional biomarker-screening method of ELISA is the employment of labile components, such as hydrogen peroxide and horseradish peroxidase. Previously, we reported that nanoceria is able to oxidize various colorimertic dyes at acidic pH, such as TMB and AzBTS, and an assay was designed for screening the folate receptor. Herein, we show that the ability of nanoceria to oxidize a substrate can be tuned by modulating the pH. Results showed that nanoceria can oxidize the non-fluorescent substrate ampliflu, either to the very stable fluorescent product resorufin at pH 7.0 or to the non-fluorescent resazurin at pH 4.0. Based on these findings, we conjugated Protein G to immobilize antibodies on the surface of nanoceria, in order to detect the expression of prototypic cancer biomarkers at pH 7.0, such as the folate receptor and EpCAM. We found that within 3 h, nanoceria identified the expression of the folate receptor and EpCAM on lung carcinoma and breast adenocarcinoma cells respectively. Traditional ELISA had a readout time of 15 h and a higher detection threshold, while requiring multiple washing steps. Considering these results and nanoceria’s ability to oxidize ampliflu to its stable fluorescent product at neutral pH, the use of antibody-carrying nanoceria in the lab and point-of-care molecular diagnostics is anticipated.
登录
查看更多内容
影响因子：
17.1
作者：
Mani, Vigneshwaran;Chikkaveeraiah, Bhaskara V.;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
7.4
作者：
Ambrosi, Adriano;Airo, Federico;Merkoci, Arben
通讯作者：
Merkoci, Arben
DOI：
10.1016/0167-4838(90)90014-7
发表时间：
1990-03-29
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ARNAO, MB;ACOSTA, M;GARCIACANOVAS, F
通讯作者：
GARCIACANOVAS, F
影响因子：
16.1
作者：
Kaittanis, Charalambos;Santra, Santimukul;Perez, J. Manuel
通讯作者：
Perez, J. Manuel
影响因子：
5.7
作者：
Winkler J;Martin-Killias P;Plückthun A;Zangemeister-Wittke U
通讯作者：
Zangemeister-Wittke U