Characterization of self-assembled virus-like particles of Merkel cell polyomavirus.
Characterization of self-assembled virus-like particles of Merkel cell polyomavirus.
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DOI：
10.1371/journal.pone.0115646
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Suzuki T
中科院分区：
文献类型：
--
作者：
Li TC;Iwasaki K;Katano H;Kataoka M;Nagata N;Kobayashi K;Mizutani T;Takeda N;Wakita T;Suzuki T
在我们的重组杆状病毒系统中，与Merkel细胞癌相关的Merkel细胞多瘤病毒(MCPyV)的VP1蛋白在昆虫细胞中自组装成两个不同大小的MCPyV样粒子(MCPyV-LP)，然后释放到培养基中。来自寄主昆虫细胞的1.5-5kb的DNA分子被包装在大的~50 nm的球形颗粒中，而不是包装在小的~25 nm的颗粒中。冷冻电子显微镜的结构重建表明，大的MCPyV-LP由72个五聚体胶囊组成，排列在T=7的二十面体表面晶格中，直径为48 nm。MCPyV-LP与BK-和JC病毒(BKPyV和JCPyV)没有相同的抗原决定簇。以VLP为基础的酶免疫测定法被应用于调查日本1-70岁人群中MCPyV感染的特定年龄段的患病率。MCPyV在儿童和青年人群中的血清阳性率随年龄增长而升高，但各年龄组的MCPyV血清阳性率均低于BKPyV和JCPyV。
In our recombinant baculovirus system, VP1 protein of merkel cell polyomavirus (MCPyV), which is implicated as a causative agent in Merkel cell carcinoma, was self-assembled into MCPyV-like particles (MCPyV-LP) with two different sizes in insect cells, followed by being released into the culture medium. DNA molecules of 1.5- to 5-kb, which were derived from host insect cells, were packaged in large, ~50-nm spherical particles but not in small, ~25-nm particles. Structure reconstruction using cryo-electron microscopy showed that large MCPyV-LPs are composed of 72 pentameric capsomeres arranged in a T = 7 icosahedral surface lattice and are 48 nm in diameter. The MCPyV-LPs did not share antigenic determinants with BK- and JC viruses (BKPyV and JCPyV). The VLP-based enzyme immunoassay was applied to investigate age-specific prevalence of MCPyV infection in the general Japanese population aged 1–70 years. While seroprevalence of MCPyV increased with age in children and young individuals, its seropositivity in each age group was lower compared with BKPyV and JCPyV.
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影响因子：
11.8
作者：
Mizutani, Tetsuya;Endoh, Daiji;Nishimura, Hidekazu
通讯作者：
Nishimura, Hidekazu
影响因子：
--
作者：
Nicol, Jerome T. J.;Robinot, Remy;Coursaget, Pierre
通讯作者：
Coursaget, Pierre
影响因子：
64.8
作者：
LIDDINGTON, RC;YAN, Y;HARRISON, SC
通讯作者：
HARRISON, SC
DOI：
10.1038/nrmicro2992
发表时间：
2013-04
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.4
作者：
Li, PP;Nakanishi, A;Kasamatsu, H
通讯作者：
Kasamatsu, H